中国动物传染病学报2017，Vol.25Issue(2)：9-13,5.
兔出血症病毒特异性核酸适配体的筛选
SCREENING OF SPECIFIC APTAMERS FOR RABBIT HEMORRHAGIC DISEASE VIRUS
摘要
Abstract
In the present study, a random oligonucleotide library (80 nt) was constructed to select the aptamers against capsid protein VP60 of Rabbit hemorrhagic disease virus (RHDV) and develop a new diagnostic method. Post SDS-PAGE separation, protein VP60 was transferred onto PVDF membranes. After 20 rounds of screening, the secondary library was obtained, whose affinity and specificity were detected by using biotin-labeled aptamers as the primary antibody and HRP-streptavidin as the secondary antibody. The results showed that VP60 was identified by biotin-labeled subordinated library. In conclusion, the recombinant plasmids were cloned, sequenced, detected and eventually three specific nucleotide sequences were obtained.关键词
兔出血症病毒/适配体/SELEX技术Key words
Rabbit hemorrhagic disease virus/aptamers/SELEX分类
农业科技引用本文复制引用
焦美会,刘家森,姜骞,陆涛峰,胡小亮,蒋艳妹,曲连东..兔出血症病毒特异性核酸适配体的筛选[J].中国动物传染病学报,2017,25(2):9-13,5.基金项目
中国农业科学院创新团队科研项目 （）
