中国现代医学杂志2017，Vol.27Issue(9)：1-7,7.DOI:10.3969/j.issn.1005-8982.2017.09.001
天麻素抗心肌氧化应激损伤的作用机制研究
Protective effect of gastrodin against myocardial oxidative stress damage
摘要
Abstract
Objective To investigate whether gastrodin plays a protective role in rat myocardial cell oxida-tive stress by mitochondrial pathway. Methods H2O2 (650μmol/L) was used to induce oxidative stress injury of H9c2 cells, and the pretreatment concentration of gastrodin was 50.0, 10.0, 1.0 and 0.1μmol/L respectively. Laser scanning confocal microscopy (LSCM) was used to detect mitochondrial membrane potential. MTT method was used to test the effect of gastrodin on cell survival rate. Western blot was used to observe the influences of gastrodin on the activity of glycogen synthase kinase 3β (GSK-3β) and protein kinase B (Akt). Results Pretreatment of gastrodin with different concentrations could prevent the reduction of fluorescence intensity of tetramethylrhodamine ethyl ester caused by H2O2, and the 10.0 μmol/L gastrodin pretreatment group had the most obvious effect compared with the model group [(0.79 ± 0.08) vs (0.30 ± 0.25)]. Pretreatment with gastrodin(10.0μmol/L) improved the survival rate of H9c2 cells (P<0.05) and increased protein expressions of p-GSK-3β (Ser9) and p-Akt (Ser473); whereas PI3K inhibitor, Wortmannin penicillin (Wort), could block this effect. Conclusions Gastrodin can alleviate oxidative stress damage of H9c2 myocardial cells caused by H2O2. It may inactivate GSK-3β through PI3K/Akt pathway, thereby play a cardiac-protective role through inhibition of mPTP opening.关键词
天麻素/线粒体通透性转换孔/氧化应激损伤/磷脂酰肌醇-3激酶/蛋白激酶-BKey words
gastrodin/mitochondrial permeability transition pore/oxidative stress injury/phosphatidylinos-itol-3 kinase/protein kinase B(Akt)分类
医药卫生引用本文复制引用
郝帅林,田炜,张媛,魏艳杰,李朝阳,肖童,苏琦,苗雨欣,徐菁蔓,郝志梅..天麻素抗心肌氧化应激损伤的作用机制研究[J].中国现代医学杂志,2017,27(9):1-7,7.基金项目
河北省自然科学基金(No:H2012401036) （No:H2012401036）
河北省高等学校科学技术研究项目(No:QN20131072) （No:QN20131072）
华北理工大学大学生创新项目(No:X2016214) （No:X2016214）
