中国现代医学杂志2017，Vol.27Issue(9)：8-12,5.DOI:10.3969/j.issn.1005-8982.2017.09.002
Akt/GSK-3β通路介导调控莱菔硫烷减轻心脏移植缺血再灌注损伤的研究
Sulforaphane preconditioning attenuates myocardial cold ischemia reperfusion injury of heart transplantation of rats through Akt/GSK-3β signaling pathway*
摘要
Abstract
Objective To explore the role of Akt/Gsk-3β signaling pathway in sulforaphane (SFN) protecting rats from cold myocardial ischemia-reperfusion injury during heart transplantation. Methods Sixty-four health male Sprague-Dawley (SD) rats were randomly divided into 4 groups, i.e. ischemia reperfusion injury group (IRI group), sulforaphane group (SFN group), LY294002+ ischemia reperfusion injury (LY+IRI group), and LY294002+sulforaphane group (LY+SFN group). Isogeneic heterotopic heart transplantation model was estab-lished according to Li's method. Donor hearts storaged in histidine-tryptophan-ketoglutarate solution for 9 h were transplanted into the abdominal cavities of recipient rats to establish rat models of heterotopic heart transplantation. Akt, p-Akt, GSK-3β and p-GSK-3β protein expressions in the grafts were detected by im-munohistochemistry and Western bolt 24 h after reperfusion. Results Compared with the IRI group, p-Akt and p-GSK-3β levels increased in the SFN group (P<0.05). After using blocker LY294002, there was no signifi-cant difference in the expression of p-Akt or p-GSK-3β between the LY+SFN group and the LY+IRI group (P>0.05). Conclusions SFN reduces cold ischemia reperfusion injury in heart transplantation of rats through activating Akt/ GSK-3β signaling pathway.关键词
蛋白激酶B/糖原合成酶激酶-3β通路/莱菔硫烷/心脏移植/冷缺血再灌注损伤Key words
Akt/Gsk-3β signaling pathway/sulforaphane/heart transplantation/cold ischemia reperfusion injury分类
医药卫生引用本文复制引用
伊雪,李占清,杨学慧,杨述亮,石莺,邬鹏宇,王耕银,陈乃耀,高木火,高毅哲..Akt/GSK-3β通路介导调控莱菔硫烷减轻心脏移植缺血再灌注损伤的研究[J].中国现代医学杂志,2017,27(9):8-12,5.基金项目
福建省自然科学基金(No:2016D014) （No:2016D014）
福建省医学创新课题(No:2015-CXB-47) （No:2015-CXB-47）
福建省厦门市科技惠民项目(No:3502Z20164057) （No:3502Z20164057）
