中国现代医学杂志2017，Vol.27Issue(9)：19-24,6.DOI:10.3969/j.issn.1005-8982.2017.09.004
臭氧氧化后处理对肾脏缺血再灌注损伤的作用
Ozone oxidative post-conditioning protects rat kidney from ischemia reperfusion injury
陈国晓 1刘修恒 2张祥生 1丁德刚 1朱晓博 1陈鑫 1闫天中1
作者信息
- 1. 河南省人民医院(郑州大学人民医院)泌尿外科,河南 郑州 450003
- 2. 武汉大学人民医院(湖北省人民医院)泌尿外科,湖北 武汉 430060
- 折叠
摘要
Abstract
Objective To explore the effect of ozone oxidative post-conditioning on chronic renal fibrosis induced by ischemia reperfusion injury in rats and the mechanism. Methods Totally 72 adult male SD rats were randomly divided into four groups (18 in each): sham-operation control group (S group), ischemia reperfusion group (I/R group), ozone oxidative post-conditioning group (I/R+O3 group) and oxygen post-conditioning group (I/R+O2 group). Serum creatinine (Scr) and blood urea nitrogen (BUN) levels were evaluated 2 w after successful modeling. Renal specimens were obtained in the 2th and 10th week respectively, and the renal tissues were stained by HE and Masson staining. The protein expressions of transforming growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) were determined by immunohistochemistry analysis. Results After two weeks of treatment, Scr and BUN of each group basically returned to normal levels, and there were no significant differences among the four groups (P> 0.05). Ten weeks after operation, Masson staining revealed renal fibrosis of the I/R, I/R+O2 and I/R+O3 groups was more serious than that of the S group (P<0.05), renal fibrosis of the I/R+O3 group was milder than taht of the I/R and I/R+O2 groups (P<0.05), while there was no difference between the I/R group and the I/R+O2 group (P>0.05). The protein expressions of TGF-β1 and α-SMA of the I/R, I/R+O2 and I/R+O3 groups were higher than those of the S group (P<0.05);and compared with the I/R group, the expressions of TGF-β1 and α-SMA were lower in the I/R+O3 group (P<0.05). There was no difference in the expression of TGF-β1 or α-SMA between the I/R+O2 group and the I/R group (P>0.05). Conclusions Ozone oxidative post-conditioning can relieve chronic renal fibrosis caused by ischemic reperfusion injury, and inhibition of activation of TGF-β1/Smad signaling pathway may be one of the mechanisms.关键词
缺血再灌注损伤/肾纤维化/臭氧氧化后处理Key words
ischemia/reperfusion injury/renal fibrosis/ozone oxidative post-conditioning分类
医药卫生引用本文复制引用
陈国晓,刘修恒,张祥生,丁德刚,朱晓博,陈鑫,闫天中..臭氧氧化后处理对肾脏缺血再灌注损伤的作用[J].中国现代医学杂志,2017,27(9):19-24,6.
