中国现代医学杂志2017，Vol.27Issue(9)：25-29,5.DOI:10.3969/j.issn.1005-8982.2017.09.005
PPARγ1基因转染对缺血再灌注心肌细胞凋亡的作用和影响
Effects of PPAR gamma 1 gene transfection on apoptosis of cardiomyocytes after ischemia reperfusion
刘永平 1魏来 1陈文雁 1孔高茵1
作者信息
- 1. 湖南省人民医院 麻醉医学中心,湖南 长沙 410005
- 折叠
摘要
Abstract
Objective To explore the effect and influence of over-expression of peroxisome proliferator-activated receptor γ1 (PPARγ1) gene on the apoptosis of myocardial cells after ischemia reperfusion. Methods Thirty SD rats were randomly divided into three groups (10 in each group), i.e. group A (sham group), group B (myocardial ischemia-reperfusion group) and group C (PPARγ1 gene group). Myocardial tissues were trans-fected with recombinant adenovirus vector mediated enhanced green fluorescent protein (Ad-EGFP) via coro-nary artery in the groups A and B. Myocardial tissues were transfected with recombinant adenovirus vector mediated PPARγ1 gene (Ad-PPARγ1) in the group C. In the group A the coronary artery was crossed through with a line without ligation; while myocardial ischemia-reperfusion injury (ischemia 30 min, reperfusion 120 min) was induced in the groups B and C three days later. Under electron microscope the changes of the myocar-dial ultrastructures were observed. Bcl-2 and Bax expressions in the heart were detected by Western blot. TUNEL method was used to measure myocyte apoptosis. Results After ischemia and reperfusion, the cardio-my ocytes in the group B were injuried most seriously under electron microscope; the myocardial fibers were arranged irregularly, ruptured and dissolved; the mitochondria were swellon with fuzzy structure and partial cristae fragmentation and dissolution. The subcellular structure damage in the group C was milder than that of the group B. Compared with the group A, Bcl-2/Bax ratio decreased significantly in the group B (P<0.05), but had no significant change in the group C (P>0.05). Compared with the group B, Bcl-2/Bax ratio in the group C increased significantly (P<0.05). The number of apoptotic cardiomyocytes in the groups B and C was significantly larger than that in the group A (P<0.05). The number of myocardial apoptosis in the group B was significantly larger than that in the group C (P<0.05). Conclusions Over-expression of PPARγ1 gene can protect myocardial tissues from ischemia reperfusion injury by reducing oxidative stress and cell apoptosis.关键词
心肌缺血再灌注损伤/过氧化物酶体增殖物激活受体γ1/细胞凋亡Key words
myocardial ischemia-reperfusion injury/peroxisome proliferator-activated receptor γ1/cell apoptosis分类
医药卫生引用本文复制引用
刘永平,魏来,陈文雁,孔高茵..PPARγ1基因转染对缺血再灌注心肌细胞凋亡的作用和影响[J].中国现代医学杂志,2017,27(9):25-29,5.
