摘要
Abstract
Objective To investigate the effect of TNF-α, IL-1β and LPS on proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) in vitro, so as to further study the mechanism of endo-thelial cell injury induced by inflammatory cytokines in different period after eye burn. Methods The HUVECs were randomly divided into control group, TNF-α group, IL-1β group and LPS group. Cell proliferation acti-vity was assessed by CCK-8. The number of proliferative cells was detected by trypan blue exclusion staining. The apoptosis was assessed by AO-EB staining. The apoptosis rate was detected by flow cytometry. Results Compared to the control group, the cell proliferation activity in the other 3 groups were markedly decreased in a downward trend from TNF-α group, IL-1β group to LSP group. Meanwhile, the results of proliferative cell counting were also similar with CCK-8 results. The cell structures in the control group were normal, and apoptotic cells were observed in the other 3 groups. Compared with the control group, the apoptosis rate of HUVECs in the TNF-α, IL-1β and LPS groups was significantly increased; and the cell apoptosis rate of the LPS group was markedly higher than that of the TNF-α and IL-1β groups, and the cell apoptosis rate of the IL-1β group was markedly higher than that of the TNF-α group (P<0.05). Conclusions The inflammatory cytokines such as TNF-α, IL-1β and LPS in early and late stages after burn can significantly decrease pro-liferative activity of HUVECs and increase the apoptosis rate in different degrees. This may be related to dif-ferent signaling pathways activated by different inflammatory mediators.关键词
眼烧伤/肿瘤坏死因子-α/白介素1β/脂多糖/内皮细胞Key words
eye burn/tumor necrosis factor-α/interleukin-1β/lipopolysaccharide/endothelial cell分类
医药卫生
