中国现代医学杂志2017，Vol.27Issue(9)：39-43,5.DOI:10.3969/j.issn.1005-8982.2017.09.008
PRT318对胶原诱导人血小板Syk磷酸化的影响
Effect of PRT318 on collagen-induced Syk phosphorylation in human platelets*
摘要
Abstract
Objective To observe the effect of PRT060318 (PRT318), an inhibitor of spleen tyrosine ki-nase (Syk), on human platelet aggregation and Syk phosphorylation in vitro. Methods The effect of PRT318 on platelet aggregation induced by collagen, adenosine diphosphate (ADP) and arachidonic acid (AA) was measured by turbidimetry. The phosphorylation of Syk525/6 was detected by Western blot. Results The Syk-specific inhibitor PRT318 suppressed collagen-induced platelet aggregation, while it did not have significantly inhibitory effect on ADP- or AA-induced platelet aggregation. The present data showed that PRT318 promoted the phosphorylation of Syk525/6 in collagen-induced platelets. PP2, tyrosine protein kinase (Src) inhibitor, inhibited collagen-induced platelet aggregation and decreased the level of Syk525/6 phosphorylation induced by collagen. PP2 also blocked PRT318-enhanced Syk525/6 phosphorylation in collagen-treated platelets. Conclusions PRT318 significantly inhibits collagen-induced platelet aggregation and promotes collagen-induced phosphorylation of Syk525/6 locating in the downstream of glycoprotein VI (GPVI) signal pathway, suggesting that PRT318 may inhibit platelet activation through regulating Syk525/6 phosphorylation.关键词
PRT318/胶原/血小板集聚/Syk525/6磷酸化Key words
PRT318/collagen/platelet aggregation/phosphorylation of Syk525/6分类
医药卫生引用本文复制引用
吴鸿,韩勇军,高水波,雷震,王振涛,韩丽华..PRT318对胶原诱导人血小板Syk磷酸化的影响[J].中国现代医学杂志,2017,27(9):39-43,5.基金项目
国家自然科学基金(No:81072968) （No:81072968）
教育部留学回国人员科研启动基金[No:教外司(20011568)] （20011568）
河南省重点科技攻关项目(No:102102310334) （No:102102310334）
