摘要
Abstract
Objective To evaluate the application value of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technique in the rapid identification of rare non-fermentative bacteria. Methods From July 2013 to October 2016, 67 clinical isolates of rare non-fermentative bacteria were collected from the First Affiliated Hospital of Anhui Medical University, which included 21 strains isolated from sputum, 13 strains from urine, 9 strains from blood, 9 strains from bone marrow, 8 strains from secre-tions, 2 strains from peritoneal dialysis fluid, 1 strain from cerebrospinal fluid, 1 strain from bile, and 3 strains from other body fluids. According to the gold standard of 16SrRNA sequence analysis, the accuracy of the identification system of MALDI-TOF MS and Vitek2 Compact was compared. Results In the 67 strains of bacteria, MALDI-TOF MS technique identified 66 strains (98.5%) to species level and 1 strains (1.5%) to genus level; while Vitek2 Compact identified 59 strains (88.1%) to species level, and 8 strains (11.9%) failed to identify. 16SrRNA gene fragments were amplified from the 67 strains of experimental bacteria and success-fully sequenced, all of the strains were identified to species level. Using 16SrRNA sequence analysis as the gold standard, the accuracy of MALDI-TOF MS system and Vitek2 Compact system for identification at the species level was 98.5% (66/67) and 88.1% (59/67) respectively. Conclusions Compared with traditional bac-teria biochemical reaction method, MALDI-TOF MS technique is simple, rapid and inexpensive. Its accuracy of identification result is highly consistent with that of 16SrRNA sequence analysis, MALDI-TOF MS tech-nique can improve the accuracy of clinical identification of non-fermentative bacteria.关键词
基质辅助激光解析电离飞行时间质谱/非发酵菌/鉴定Key words
matrix assisted laser desorption ionization time of flight mass spectrometry/non-fermentative bacteria/identification分类
医药卫生
