中成药2017，Vol.39Issue(5)：890-895,6.DOI:10.3969/j.issn.1001-1528.2017.05.002
雪荔方总黄酮对LPS致RAW264.7细胞炎症的保护作用
Protective effects of total flavones from Xueli Formula on LPS-stimulated RAW264.7 cell inflammation
摘要
Abstract
AIM To observe the anti-inflammatory effects of total flavones from Xueli Formula (Violae Herba,Serissa japonica,Plantaginis Herba and Salvia plebeia,TFXL) on LPS-stimulated RAW264.7 macrophages.METHODS The effects of different concentrations of TFXL on RAW264.7 cell viability were examined by MTT assay.The NO kit assay was adopted to detect the NO release amount of TFXL on LPS-stimulated RAW264.7 cells.The secretions of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and interleukin-10 (IL-10) were tested by enzyme linked immunosorbent assay (ELISA).Reverse transcriptase-polymerase chain reaction (RTPCR) was used to determine the expressions of inducible nitric oxide synthase (iNOS),TNF-α,IL6 and IL10 mRNA.The protein expressions of IκB-α and p65 were tested by Western blot.RESULTS Compared with the LPS model group,TFXL could significantly reduce the secretions of NO,TNF-α and IL-6;increase the secretion of IL-10;inhibit the expressions of iNOS,TNF-α and IL6 mRNA;promote the expression of IL10 mRNA;inhibit the phosphorylations of IκB-α and p65.The TFXL high-dose group could inhibit the degradation of IκB-α.CONCLUSION This study preliminarily proves the protective effects of TFXL on LPS-stimulated RAW264.7 cell inflammation,whose action mechanism may be related to NF-κB signal pathway.关键词
雪荔方/总黄酮/小鼠巨噬细胞系/脂多糖/抗炎Key words
Xueli Formula/total flavones/mice macrophage system/LPS/anti-inflammation分类
医药卫生引用本文复制引用
丛友权,韩晶晶,方芸,陈军..雪荔方总黄酮对LPS致RAW264.7细胞炎症的保护作用[J].中成药,2017,39(5):890-895,6.基金项目
江苏省中医药管理局项目(HZ1016KY) （HZ1016KY）
