中成药2017，Vol.39Issue(5)：896-901,6.DOI:10.3969/j.issn.1001-1528.2017.05.003
胃炎1号方对胃癌前病变大鼠胃黏膜上皮细胞缺氧及缺氧耐受的影响
Effects of No.1 Weiyan Decoction on hypoxia and hypoxic tolerance in gastric mucosal epithelial cells in rats with gastric precancerous lesion
摘要
Abstract
AIM To study the effects of No.1 Weiyan Decoction (Codonopsis Radix,Paeoniae Radix alba,Taraxaci Herba,etc.) on hypoxia and hypoxic tolerance in gastric mucosal epithelial cells in rats with precancerous lesion of gastric cancer (PLGC).METHODS Forty rats were randomly divided into normal,model,Vitacoenzyme Tablets and No.1 Weiyan Decoction groups.Except for the normal group,the rats in the other groups were given with N-methyl-N'-nitro-N-nitrosoguanidine and Little Chengqi Decoction to establish a PLGC rat model.Ten weeks after the administration,the gastric mucosal epithelial microvascular ultrastructure was observed,the expressions of Hif1a mRNA,HIF-1 α protein and VEGF protein in gastric mucosal epithelium were detected.RESULTS Compared with the normal group,the number of microvasculatures in gastric mucosal epithelium was decreased;lumen was severely stenosed or expansive;inner wall was not smooth and had adhesion;vascular endothelial cells were swollen;the expression of Hif1α mRNA showed an increasing trend;the expressions of HIF-1α protein and VEGF protein were anomalous in the model group.Compared with the model group,the gastric mucosal vascular lesion of PLGC rats was improved,and the expressions of Hif1a mRNA,HIF-1α protein and VEGF protein were significantly decreased in the No.1 Weiyan Decoction group.CONCLUSION No.1 Weiyan Decoction can improve hypoxia and hypoxia tolerance,and induce angiogenesis in gastric mucosal epithelium in PLGC rats.关键词
胃炎1号方/胃癌前病变/胃黏膜上皮/微血管超微结构/缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)Key words
No.1 Weiyan Decoction/gastric precancerous lesion/gastric mucosal epithelium/microvascular ultrastructure/HIF-1α/VEGF分类
医药卫生引用本文复制引用
张成哲,蔡大可,卓俊城,蔡甜甜,赵自明,潘华峰,陈玉兴,曾晓会,黄雪君,姚楠..胃炎1号方对胃癌前病变大鼠胃黏膜上皮细胞缺氧及缺氧耐受的影响[J].中成药,2017,39(5):896-901,6.基金项目
国家自然科学基金项目(81273739,81473620) （81273739,81473620）
