中成药2017，Vol.39Issue(5)：902-906,5.DOI:10.3969/j.issn.1001-1528.2017.05.004
黄芪、水蛭有效成分对大鼠肾小球系膜细胞凋亡的影响
Influences of Astragalus and Hurido effective components on apoptosis of rat glomerular mesangial cells
摘要
Abstract
AIM To investigate the influences of Astragalus and Hurido effective components on apoptosis of rat glomerular mesangial cells.METHODS The proliferation of rat mesangial cells was induced by LPS and then intervened with Astragalus (astragalus polysaccharide,astragaloside Ⅳ,calycosin) and Hurido (hirudin).The cells were collected after 24 h.4',6-Diamidino-2-phenylindole (DAPI) staining was used to observe cell nuclear changes.Quantitative and qualitative expressions of Bcl-2 were detected by streptavidin-biotin complex (SABC).RESULTS Astragalus and Hurido effective components could induce the apoptosis of rat glomerular mesangial cells,and decrease the expression of Bcl-2.CONCLUSION Astragalus and Hurido effective components can induce the mesangial cell apoptosis by down-regulating Bcl-2 expression,which can be used for the treatment of mesangial proliferative glomerulonephritis.关键词
黄芪/水蛭/有效成分/细胞凋亡/系膜细胞/Bcl-2Key words
Astragalus/Hurido/effective components/cells/mesangial cells/Bcl-2分类
医药卫生引用本文复制引用
姜梦真,任惠娟,徐蕾,任现志..黄芪、水蛭有效成分对大鼠肾小球系膜细胞凋亡的影响[J].中成药,2017,39(5):902-906,5.基金项目
国家自然科学基金面上项目(81173298) （81173298）
