中成药2017，Vol.39Issue(5)：960-964,5.DOI:10.3969/j.issn.1001-1528.2017.05.015
HPLC法同时测定六合定中丸中6种成分
Simultaneous determination of six constituents in Liuhe Dingzhong Pills by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol in Liuhe Dingzhong Pills (Aurantii Fructus,Citri reticulatae Pericarpium,Magnoliae officinalis Cortex,etc.).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Dikma Spursil C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 283 nm and 294 nm.RESULTS Six constituents showed good liner relationships within their own ranges (r≥0.999 3),whose average recoveries were 92.6％-103.7％ with the RSDs of 0.89％-2.87％.The contents of naringin and neohesperidin in three batches of samples showed obvious differences.CONCLUSION We should pay attention to the unstable quality of Liuhe Dingzhong Pills due to Aurantii Fructus.关键词
六合定中丸/芸香柚皮苷/柚皮苷/橙皮苷/新橙皮苷/和厚朴酚/厚朴酚/HPLCKey words
Liuhe Dingzhong Pills/narirutin/naringin/hesperidin/neohesperidin/honokiol/magnolol/HPLC分类
医药卫生引用本文复制引用
谢淑桐,高晓霞,宋粉云,钟兆健..HPLC法同时测定六合定中丸中6种成分[J].中成药,2017,39(5):960-964,5.基金项目
上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001) （中药）
