浙江医学2017，Vol.39Issue(7)：505-507,511,4.DOI:10.12056/j.issn.1006-2785.2017.39.7.2015-1401
非小细胞肺癌患者DC-CIK过继免疫治疗前后外周血TLR4表达的变化
Changes of TLR4 mRNA expression in peripheral blood of patients with non-small cell lung cancer after DC-CIK immunotherapy
王细勇 1袁琳娜 1张志豪1
作者信息
- 1. 314000 武警浙江总队嘉兴医院胸心外科
- 折叠
摘要
Abstract
Objective To investigate the changes of TLR4 mRNA expression in peripheral blood mononuclear cell (PBMC) of patients with non-small cell lung cancer(NSCLC) after DC-CIK immunotherapy.Methods The mRNA expression of TLR4 in PBMC was detected by reverse transcription PCR (PT-PCR) in 50 patients with NSCLC before and after DC-CIK therapy.The secretion of cytokines (IL-6,IL-8 and TNF-α) of PBMC stimulated with LPS was detected by ELISA before and after DC-CIK therapy.Results After DC-CIK treatment,the relative expression level of TLR4 mRNA in PBMC of NSCLC patients was significantly decreased(t=6.272,P＜0.01),the secretion of IL-6(t=17.407,P＜0.01),IL-8 (t=15.244,P＜0.01) and TNF-α (t=12.428,P＜0.01) by PBMC stimulated with LPS was significantly decreased.Conclusion DC-CIK immunotherapy can down-regulate TLR4 expression in PBMC of NSCL patients to enhance the anti-tumor immunity.关键词
树突状细胞/细胞因子诱导的杀伤细胞/非小细胞肺癌/TLR4Key words
Dendritic cells(DC)/Cytokine-induced killer(CIK)/Non-small cell lung cancer/TLR4引用本文复制引用
王细勇,袁琳娜,张志豪..非小细胞肺癌患者DC-CIK过继免疫治疗前后外周血TLR4表达的变化[J].浙江医学,2017,39(7):505-507,511,4.
