浙江医学2017，Vol.39Issue(7)：515-517,3.DOI:10.12056/j.issn.1006-2785.2017.39.7.2017-21
大黄素对急性肝功能衰竭大鼠肝脏的保护作用机制
Effects of emodin on liver function in rats with acute liver failure and its mechanism
摘要
Abstract
Objective To investigate the effect of emodin on liver function in acute hepatic failure and its mechanism.Methods SD rats were randomly divided into three groups:healthy control group (n=6),acute liver failure (ALF) model group (n=24) and emodin treatment group (n=24).Acute liver failure was induced by intraperitoneal injection of D-galactosamine (D-Gal).Twelve hours after the model was established,the emodin group received intraperitoneal injection of 10mg/kg emodin twice daily.At 24,72,120 and 168h after modeling,6 rats were sacrificed in ALF group and emodin group.The portal vein blood samples were collected and hepatic function was examined.Liver tissue was fixed with 10％ formalin,and the expression of NF-κB in liver tissue was detected by immunohistochemistry.Results After 24h of modeling,the levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were significantly higher than those in healthy control group (P＜0.01),after 72h of modeling,the liver function of rats in ALF group was significantly higher than of emodin group at the same time point (P＜0.01),after 24h of modeling,the expression of NF-κB in ALF group was significantly higher than that in healthy control group (P＜0.01),reached the peak at 72h,then decreased after 120h.The expression of NF-κB in emodin group was significantly lower than that in ALF group at 72,120 and 168h after modeling (P＜0.01).Conclusion Emodin has protective effect on acute liver failure in rats,which may be related to the down-regulation of NF-κB expression.关键词
急性肝功能衰竭/大黄素/核因子-κB/大鼠Key words
Acute liver failure/Emodin/NF-κB/Rat引用本文复制引用
于盈,王凤玲..大黄素对急性肝功能衰竭大鼠肝脏的保护作用机制[J].浙江医学,2017,39(7):515-517,3.基金项目
浙江省中医药科学研究基金计划项目(2010ZA118) （2010ZA118）
台州市椒江区科技计划项目(112070) （112070）
