浙江医学2017，Vol.39Issue(8)：599-600,605,3.DOI:10.12056/j.issn.1006-2785.2017.39.8.2016-1016
BIIB021对MDS细胞株Skm-1增殖的多靶点抑制作用研究
BIIB021 inhibits proliferation of myelodysplastic syndrome Skm-1 cells and its mechanism
摘要
Abstract
Objective To investigate the effect of HSP-90 inhibitor BIIB021 on the proliferation of myelodysplastic syndrome(MDS) Skm-1 cells and related mechanisms.Methods Skm-1 cells were treated with 100,200 and 400nmol/l BIIB021 for 24h.The expression of proteins mTOR,mTORC1,HIF-1α in Skm-1 cells was examined by Western blot.Results The expression of protein mTOR in Skm-1 cells was not significantly changed after treatment with different concentrations of BIIB021 (P ＞0.05).Compared to control group,the expression of mTORC1 and HIF-1α in Skm-1 cells treated with different concentrations of BIIB021 was significantly decreased (P＜0.05),and the expression was the lowest in the group of 400 nmol/L.Conclusion BIIB021 inhibits proliferation of MDS Skm-1 cells through down-regulating the expression of mTORC1 and HIF-1α.关键词
HSP-90/骨髓增生异常综合征/mTORC1/HIF-1αKey words
HSP-90/MDS/mTORC1/HIF-1α引用本文复制引用
李静,沈益民,林圣云,陈珍珍..BIIB021对MDS细胞株Skm-1增殖的多靶点抑制作用研究[J].浙江医学,2017,39(8):599-600,605,3.
