浙江医学2017，Vol.39Issue(8)：606-609,613,5.DOI:10.12056/j.issn.1006-2785.2017.39.8.2016-1678
高糖腹膜透析液对HPMECs中GLUT1、SGLT1、TGF-β1、VEGF-A、CTGF表达的影响研究
Expression of GLUT1, SGLT1, TGFβ-1, VEGF-A, CTGF in human peritoneal microvascular endothelial cells cultured with high glucose peritoneal dialysis fluid
摘要
Abstract
Objective To investigate the effect of high glucose-based peritoneal dialysis fluid (PDF) on the expression of glucose transporters1 (GLUT1),Na+/glucose cotransporters1 (SGLT1),transforming growth factor-β1 (TGF-β1),vascular endothelial growth factor-A (VEGF-A) and connective tissue growth factor (CTGF) in human peritoneal microvascular endothelial cells (HPMECs).Methods The cultured HPMECs were divided into 5 groups:normal group (N),peritoneal dialysis (containing 2.5％ glucose) group (P);GLUT1 antagonists group(GN),SGLT1 antagonists group (SN);GLUT1 antagonists+ SGLT1 antagonists group (GN+SN).The mRNA and protein expression of GLUT1,SGLT1,TGF-β1,VEGF-A and CTGF in HPMECs were detected by RT-qPCR and Western blotting,respectively.Results There were significant differences in mRNA and protein expression of GLUT1,SGLT1,TGF-β 1,VEGF-A and CTGF among 5 groups (P＜0.05).Compared with group N,the mRNA and protein expression of GLUT1,SGLT1,TGF-β1,VEGF-A and CTGF in group P was increased significantly (P＜0.05).Compared with group P,the mRNA and protein expression of GLUT1,SGLT1,TGF-β1,VEGF-A and CTGF in groups GN,SN and GN+SN was decreased significantly (P＜0.05).Conclusion High glucose peritoneal dialysis fluid may up-regulate the expression of GLUT1,SGLT1,which may further induce the up-regulating expression of TGF-β1,VEGF-A and CTGF in human peritoneal microvascular endothelial cells,resulting in the peritoneal fibrosis.关键词
腹膜微血管内皮细胞/葡萄糖转运蛋白1/钠-葡萄糖共转运载体1/细胞因子转化生长因子-β1/血管内皮生长因子-A/结缔组织生长因子Key words
Peritoneal microvascular endothelial cells/Glucose transporters1/Na+/glucose cotransporters1/TGF-β1/VEGF-A/CTGF引用本文复制引用
聂振禹,洪梦琪,包蓓艳,李国富,陈争跃,余雄伟..高糖腹膜透析液对HPMECs中GLUT1、SGLT1、TGF-β1、VEGF-A、CTGF表达的影响研究[J].浙江医学,2017,39(8):606-609,613,5.基金项目
浙江省自然科学基金资助项目(Y13H050021) （Y13H050021）
宁波市科技计划项目(2015C50001) （2015C50001）
宁波市鄞州区科技计划项目(鄞科[2011]69号-26) （鄞科[2011]69号-26）
