作物学报2017，Vol.43Issue(5)：648-657,10.DOI:10.3724/SP.J.1006.2017.00648
水稻斑点叶突变体splZ97的生理特性及其基因定位
Physiological Characters and Gene Mapping of a Spotted-leaf Mutant splZ97 in Rice
摘要
Abstract
A spotted-leaf mutant splZ97 was isolated from a mutant bank generated by EMS mutagenesis of indica restore lineZhen 97. Under field conditions, the brown lesion-mimics mutant splZ97 firstly displayed at the tip and edge of leaf blade at tillering stage, and then gradually spread to whole leaf, resulting in the death of the whole blade when the symptom was severe. At the same time, the major agronomic traits including plant height, grain number per panicle and seed-setting rate were markedly affected. Compared with the wild-type the flag leaf, the second leaf from top and the third leaf from top at heading stage, chlorophyll contents in the mutant splZ97 significantly decreased, while POD (peroxidase, POD) activity, O2 ? level and MDA (malondialdehyde, MDA) content increased. In addition, CAT (catalase, CAT) activity and soluble protein content of the second leaf from top and the third leaf from top of the mutant decreased as compared with the wild type; on the contrary, the SOD (superoxide dismutase, SOD) activity significantly increased. The histochemical analysis further indicated that O2 ? accumulated in the leaf blade of the mutant splZ97. In addition, under salt stress at seedling stage, the shoot length and root length of the mutant splZ97 were significantly shorter than these of the wild type. Genetic analysis and gene mapping showed that splZ97 was controlled by a single recessive nuclear gene, which was mapped to a region of 189 kb flanked by two SSR markers RM28466 and RM28485 on the long arm of chromosome 12. These results achieved in the present study would further facilitate the cloning and functional analysis of the gene SPLZ97.关键词
水稻/splZ97/斑点叶/生理特性/基因定位Key words
Rice/splZ97/Spotted-leaf/Physiological characters/Gene mapping引用本文复制引用
韦荔全,罗延敏,王文强,池长程,黄福灯,向珣,程方民,潘刚..水稻斑点叶突变体splZ97的生理特性及其基因定位[J].作物学报,2017,43(5):648-657,10.基金项目
本研究由国家自然科学基金项目(31271691)和国家转基因生物新品种培育重大专项(2016ZX08001-002)资助. This study was supported by the Natural Science Foundation of China (31271691) and the National Major Project for Developing New GM Crops (2016ZX08001-002) （31271691）
