作物学报2017，Vol.43Issue(5)：658-668,11.DOI:10.3724/SP.J.1006.2017.00658
利用II-32B/A7444组合CSSL群体定位水稻7个穗部性状QTL
Mapping QTLs for Seven Panicle Traits in Rice (Oryza sativaL.) Using Chro-mosome Segment Substitution Lines Derived from Ⅱ-32B/A7444
摘要
Abstract
For mining elite alleles controlling panicle traits in rice, a mapping population was constructed with chromosome segment substitution lines (CSSLs) by crossing and back crossing the indica variety Ⅱ-32B (receptor parent) and the japonica variety A7444 (donor parent). A total of 26 QTLs associated with seven panicle traits were detected across two years using the QTL Ici- Mapping 4.1 software, and 13 QTLs were identified repeatedly in both years. Among them, the four QTLs for primary branch number (PBN) were located on chromosomes 1, 6, 8, and 9 with average phenotypic variation explained (PVE) of 15.16%,13.10%, 29.74%, and 11.21%, and average additive effect (AE) of -1.40, 1.01, 1.11, and 0.77, respectively; the two QTLs for secondary branch number (SBN) were located on chromosomes 6 and 8 with average PVE of 10.97% and 21.39%, and average AE of 5.45 and 6.36, respectively; the three QTLs for spikelet number per panicle (SPP) were located on chromosomes 2, 6, and 8 with average PVE of .65%, 12.52%, and 31.22%, and average AE of -18.61, 22.23, and 31.87, respectively; the unique locus for grains per panicle (GPP) was located on chromosome 8 with average PVE of 28.06% and average AE of 30.85; the two QTLs for 1000-grain weight (TGW) were located on chromosome 2 with average PVE of 44.65% and 17.51%, and average AE of 2.88 and -2.51, respectively; and only one QTL for grain width (GW) was located on chromosome 10 with average PVE of 21.96% and average AE of 0.11. Segments simultaneously controlling SBN, SPP and GPP were found on chromosomes 2, 6, and 8. According to bi-parent DNA sequence comparison, QTLs qSBN6 and qSBN8 were speculated to be different from Hd1 and DTH8 because 16 and 1 base difference were found, respectively. QTL qSBN2 seems to be a novel locus that controlling secondary branch number. These results are valuable for pyramiding breeding through marker-assisted selection.关键词
水稻/染色体片段置换系/穗部性状/数量性状位点Key words
Rice/Chromosome segment substitution lines/Panicle traits/Quantitative trait locus引用本文复制引用
佘东,刘强明,李大露,梁银凤,刘二宝,党小景,洪德林..利用II-32B/A7444组合CSSL群体定位水稻7个穗部性状QTL[J].作物学报,2017,43(5):658-668,11.基金项目
本研究由高等学校博士学科点专项科研基金(20130097110001), 中央高校基本科研业务费专项基金项目(KYZ201202-9)和国家自然科学基金项目(31571743)资助. This study was supported by the Doctoral Found of Ministry of Education (20130097110001), a grant from the Ministry of Education of China for Basic Scientific Research of National Universities (KYZ201202-9), and the National Natural Science Foundation of China (31571743). （20130097110001）
