作物学报2017，Vol.43Issue(5)：669-677,9.DOI:10.3724/SP.J.1006.2017.00669
茶树越冬芽在休眠与萌发时期的物质交流变化及其分子调控
Molecular Regulation and Substance Exchange Dynamics at Dormancy and Budbreak Stages in Overwintering Buds of Tea Plant
摘要
Abstract
Early-sprouting cultivar Longjing 43 and late-sprouting cultivar Biyun were employed in this study to disclose the dormancy mechanism in tea plant with different sprouting phenophases. The levels of substance exchange were monitored by detecting the fluorescence signal in calcein treated overwintering buds. The glucanase related genes were identified by sequence homology analysis. Their characteristics and expression patterns during different time of winter were further analyzed. The sub-stance exchanges were detected either in stem-bud unit or mother leaf-stem unit. From the initial formation to release in dormancy, the substance exchange in overwintering buds showed strong-weak-strong variation patterns in both cultivars, however, the dura-tion of weak exchange stage was much shorter in Longjing 43 than in Biyun. Moreover, there was a close correlation between substance exchange variation pattern and the expression pattern of CsGLU1, a gene identified in tea plant with positive callose hydrolyzation activity. On the basis of promoter sequence analysis, plenty of transcription factor binding sequences related to hormone signaling, cold stimulation and dormancy regulation were found in CsGLU1 promoter region, which validates its puta-tive functions in dormancy regulation. In conclusion, overwintering buds of tea plant have substance exchange with stem and mother leaf both in dormancy and non-dormancy status, furthermore, the variation of substance exchange level was consistent to the changes of dormancy status.CsGLU1 is a callose hydrolyzation related gene, which is supposed to be a key gene regulating tea plant dormancy transition through affecting the substance exchange in overwintering buds. The study provides meaningful results for understanding the changes of dormancy statuses in overwintering buds and deeply exploring the regulation mechanism in tea plant with different sprouting phenophase.关键词
茶树/越冬芽休眠/物质交流/钙黄素Key words
Tea plant/Overwinter bud dormancy/Substances exchange/Calcein引用本文复制引用
唐湖,郝心愿,王璐,肖斌,王新超,杨亚军..茶树越冬芽在休眠与萌发时期的物质交流变化及其分子调控[J].作物学报,2017,43(5):669-677,9.基金项目
本研究由国家自然科学基金项目(31370690), 国家现代农业产业技术体系建设专项(CARS-23)和中国农业科学院农业科技创新工程(CAAS-ASTIP-2014-TRICAAS)资助. This study was supported by the National Natural Science Foundation of China (31370690), the China Agriculture Research System (CARS-23), and the Agricultural Science and Technology Innovation Program of CAAS (CAAS-ASTIP-2014-TRICAAS). （31370690）
