作物学报2017，Vol.43Issue(5)：691-700,10.DOI:10.3724/SP.J.1006.2017.00691
小麦突变体AS208中Glu-B1位点缺失对籽粒中蛋白体形成和储藏蛋白合成与加工相关基因表达的影响
Glu-B1 Silencing Influences Protein Body Formation and Expression of Genes Regulating Synthesis and Processing of Seed-Storage Protein in Somatic Mu-tant Wheat AS208
摘要
Abstract
High-molecular-weight-glutenin subunits (HMW-GSs) are important components of seed storage proteins in grains of wheat (Triticum aestivum L.), which determine wheat dough elasticity and processing quality. Using AS208, a wheat somatic variation line with silenced 1Bx20 and By20 at Glu-B1 locus, and its originating cultivar Lunxuan 987, we studied the present status of Glu-B1, protein body (PB) formation progress, and expression trends of four kinds of genes (BiP,Dof,PDI, and SPA) related to glutenin synthesis and accumulation at different stages of grain development. Southern blotting analysis revealed that AS208 had two specific bands less than its control parent Lunxuan 987. The fluorescencein situ hybridization (FISH) assay showed six and four chromosomes with signals in Lunxuan 987 and AS208, respectively. These results indicate that the Glu-B1 locus has been deleted from the genome of AS208. Compared to Lunxuan 987, AS208 showed similar size and shape of seed PB during PB formation under a transmission electron microscope (TEM). A total of 12 transcription-factor or molecular-chaperone genes were subjected to qRT-PCR assay. These genes were related to glutenin coding genes expression, storage proteins accumu-lation and processing, PB assembling and transportation. Seven out of 12 genes had simialr expression patterns in AS208 and Lunxuan 987, and eight geneswere expressed more in AS208 than in Lunxuan 987, especially the expression levels of Bip,PDI-1, and PDI-5 were 1.5–2.0 folds higher in AS208 than in Lunxuan 987. These results suggested that Glu-B1 silencing had no sig-nificant effect on the formation of PB during wheat grain development, but partially spurred the expression of some genes regu-lating the synthesis and processing of seed-storage proteins. As a result, via a negative feedback regulation pathway, the protein content in seed and the shape and size of PB remain in similar levels between AS208 and its originating cultivar.关键词
小麦/高分子量谷蛋白亚基/蛋白体/转录因子/分子伴侣Key words
Wheat/High molecular weight glutenin subunits/Protein body/Transcription factor/Molecular chaperones引用本文复制引用
刘会云,王婉晴,李欣,王轲,王龙,杜丽璞,晏月明,叶兴国..小麦突变体AS208中Glu-B1位点缺失对籽粒中蛋白体形成和储藏蛋白合成与加工相关基因表达的影响[J].作物学报,2017,43(5):691-700,10.基金项目
本研究由国家自然科学基金项目(31371621)资助. This study was supported by the National Natural Science Foundation of China (31371621). （31371621）
