作物学报2017，Vol.43Issue(5)：708-717,10.DOI:10.3724/SP.J.1006.2017.00708
茶树金属耐受蛋白基因CsMTP11的克隆及功能分析
Cloning and Function Analysis of Metal Tolerance Gene (CsMTP11) in Tea Plant (Camellia sinensisL. O. Kuntze)
摘要
Abstract
As a member of cation diffusion facilitator family, metal tolerance protein (MTP) is involved in the regulation of heavy metal stress process in plant. Here, we cloned a MTP gene, CsMTP11 (GenBank accession number: KX450265) from Zhongcha 108 tea plant. The full-length cDNA of CsMTP11 is 1197 bp, and the gene encodes a novel protein of 398 amino acids, which shares significant sequence similarity with VvMTP11 (90%). The molecular weight and theoretical pI of this protein are 44.85 kD and 5.34, respectively. The conserved domain analysis indicated that CsMTP11 contained five transmembrane domains and the conserved CDF domain. The phylogenetic tree indicated that CsMTP11 had sequence conservation among different species. The transcriptional level of CsMTP11 in old leaf was higher than that in root. Futher more, the heavy metal Mn and Co stresses induced the expression of CsMTP11. The subcellular localization assay using CsMTP11-YFP fusion gene expressed in the Arabidopsis protoplast showed that CsMTP11 was localized in the plasma membranes. CsMTP11 heterologous expression in wild-type yeast BY4741 and the mutants showed that it was able to increase tolerance to Mn and Co. Taken together , our results indicated that CsMTP11 is a member of the Mn-CDF family, and it may be involved in the regulation of heavy metal stress process in tea plant.关键词
茶树/重金属耐受蛋白/基因克隆/锰Key words
Camellia sinensis/Heavy metal tolerance/Gene clone/Mn引用本文复制引用
袁连玉,陈应娟,魏旭,童华荣..茶树金属耐受蛋白基因CsMTP11的克隆及功能分析[J].作物学报,2017,43(5):708-717,10.基金项目
本研究由国家自然科学基金项目(31400583), 重庆市基础与前沿研究计划一般项目(cstc2014jcyjA80011)和中央高校基本业务费专项(XDJK2014C070)资助. This work was supported by the National Natural Science Foundation of China (31400583), the Basic and Frontier Research Program of Chongqing (cstc2014jcyjA80011), and Fundamental Research Funds for Central Universities (XDJK2014C070). （31400583）
