中医药学报2017，Vol.45Issue(2)：15-18,4.
归芪多糖对衰老大鼠肾组织氧化损伤作用的研究
Effect of Guiqi Polysaccharide on Oxidative Damage of Kidney Tissue in Aging Rats
摘要
Abstract
Objective:To study the effect of Guiqi polysaccharide on oxidative damage of kidney tissue in D-galactose-induced aging rats.Methods:We extracted polysaccharide from a percentage of angelica and astragalus membranaceus through the water extraction and alcohol precipitation method,and determined the content of polysaccharide with phenol sulfuric acid method.D-galactose was subcutaneous injected at a dose of 0.15 g/kg body weight every day except the normal control group.Rats of the low-and high-dose Guiqi polysaccharide (2.0 g/kg and 0.5 g/kg) were orally administrated daily for 42 days,respectively.Then superoxide dismutase (SOD) activity,the content of glutathione peroxidase (GSH-PX),glutathione (GSH),malondialdehyde (MDA) content,protein carbonyl of kidney were determined,respectively.Results:Guiqi polysaccharides could enhance SOD,GSH-PX,GSH enzyme activity in renal tissue of aging rats,and it could significantly decrease the content of MDA and protein carbonyl.Conclusion:Guiqi polysaccharide can reduce free radical damage to the kidney of aging rats and protect the normal kidney from senescence.关键词
归芪多糖/衰老/SOD/GSH-PX/GSH/MDA/蛋白羰基Key words
Guiqi polysaccharide/Aging/SOD/GSH-PX/GSH/MDA/Protein carbonyl分类
医药卫生引用本文复制引用
蒲秀瑛,李海兵,李晓玥,马小龙,刘璐,任菁..归芪多糖对衰老大鼠肾组织氧化损伤作用的研究[J].中医药学报,2017,45(2):15-18,4.基金项目
国家自然科学基金项目(81260070) （81260070）
