中医药学报2017，Vol.45Issue(2)：50-54,5.
益母草愈伤组织诱导与生物转化能力初探
Callus Induction and Biotransformation Ability of Leonurus Japonicus
摘要
Abstract
Objective:To establish the callus culture system of Leonurus japonicus,which was used in bioconversion exogenous substrates.Methods:MS was selected as the basic medium,and the effects were detected according to callus induction and proliferation with different explants and plant growth regulators,and then the callus ability of bioconversion was explored.Results:The callus induction rate of cotyledon was 85.79％;the induction rate of true leaf was 97.88％;the induction rate of hypocotyl was 29.78％.The optimum medium formula for inducing callus was MS + 2,4-D 0.5 mg/L +6-BA 2 mg/L;that for initial proliferation was MS +2,4-D 0.5 mg/L +6-BA 2 mg/L;that for callus Long-term successive transfer culture was MS + 2,4-D 0.1 mg/L + 6-BA 1 mg/L;the optimal cycle of subculture was 10 d;the three kinds of callus in suspension culture had the ability of hydroquinone into arbutin.Conclusion:By optimizing proliferation,the callus of Leonurus japonicus is expected to become a good material for transformation exogenous compounds.关键词
益母草/愈伤组织/生物转化/氢醌/熊果苷/糖基化Key words
Leonurus japonicas/Callus/Biotransformation/Hydroquinone/Arbutin/Glycosylation分类
医药卫生引用本文复制引用
唐婕妤,罗月芳,龙靖娴,彭菲..益母草愈伤组织诱导与生物转化能力初探[J].中医药学报,2017,45(2):50-54,5.基金项目
湖南省科技厅社会发展支撑计划项目(2013SK3081) （2013SK3081）
