安徽农业科学2017，Vol.45Issue(13)：99-102,118,5.
HPLC-MS法测定饮料中甜蜜素等6种非糖类甜味剂含量
Determination of Cyclamate Sodium etc.Six Kinds of Sweetening Agent of Non-carbohydrate in the Drinks by HPLC-MS
张倩勉 1盘正华 1范兴 1邓玉秀 1庞贞武2
作者信息
- 1. 广西-东盟食品药品安全检验检测中心,广西南宁530021
- 2. 广西八桂林木种苗股份有限公司,广西南宁530021
- 折叠
摘要
Abstract
[Objective] In order to establish a rapid,accurate,high sensitivity method for determination of Cyclamate Sodium etc.six kinds of sweetening agent.[Method] The contents of Cyclamate Sodium,Acestlfame,Aspartame,Neotame,Sucralose and Stevioside in commercially available beverage were determined by HPLC-MS,the positive and negative ion modes were used to conduct MRM.[Result] The results showed that a good linearity was obtained for the standard curve with 20 to 1 000 ng/mL,and the regression equation and correlation coefficient R were:Cyclamate Sodium:Y=3 380X-49 100,R =0.999 7;Acesulfame Y=8 620X +21 700,R =0.998 7;Aspartame:Y=4 070X-12 400,R =0.999 7;Neotame:Y=5 930X-21 300,R =0.999 4;Sucralose:Y=123X +425,R =0.999 7;Stevioside:Y =332X-967,R =0.997 1.The minimum detection limits were:Cyclamate Sodium:0.1 mg/kg;Acesulfame:0.2 mg/kg;Aspartame:0.2 mg/kg;Neotame:0.03 mg/kg;Sucralose:0.2 mg/kg;Stevioside:0.2 mg/kg.[Conclusion] This method is rapid,simple,sensitive and accurate,and it is a suitable method for rapid determination of Cyclamate Sodium etc.six kinds of sweetening agent of non-carbohydrate in the food.关键词
高效液相色谱-串联质谱法/饮料/甜味剂/含量/测定Key words
HPLC-MS/Drink/Sweetening agent/Content/Determination分类
轻工纺织引用本文复制引用
张倩勉,盘正华,范兴,邓玉秀,庞贞武..HPLC-MS法测定饮料中甜蜜素等6种非糖类甜味剂含量[J].安徽农业科学,2017,45(13):99-102,118,5.
