安徽农业科学2017，Vol.45Issue(13)：143-144,150,3.
成熟油菜种子RNA提取方法的改良
Improvement of RNA Extraction Method from Mature Rapeseeds
摘要
Abstract
[Objective] To extract high quality RNA from mature rapeseeds.[Method] To improve the RNA extraction method of mature rapeseeds by using two RNA extraction kits,which include RNA plant plus reagent from TIANGEN and CW2598M from KANGWEI,meanwhile,adding β-mercaptoethanol to inhibit the oxidation of phenolic compounds and removing DNA by DNase,using the adsorption column to remove polysaccharides etc.[Result] This method can get RNA with high quality,which can satisfy the requirements of subsequent molecular biology research.[Conclusion] The study provides an efficient method for extracting RNA from mature rapeseeds.关键词
油菜/成熟种子/RNA提取Key words
Rape/Mature seeds/RNA extraction分类
农业科技引用本文复制引用
顾思凯,陈桂敏,赵文君,刘福霞,赵祥祥,纪丽莲..成熟油菜种子RNA提取方法的改良[J].安徽农业科学,2017,45(13):143-144,150,3.基金项目
国家自然科学基金项目(31470572). （31470572）
