北京中医药大学学报2017，Vol.40Issue(5)：376-384,9.DOI:10.3969/j.issn.1006-2157.2017.05.005
益气活血方对心肌缺血大鼠Akt/mTOR信号通路的影响
Influence of Yiqi Huoxue Fang on Akt/mTOR signaling pathway in rats with myocardial ischemia of myocardial
摘要
Abstract
Objective To investigate the myocardial expressions of protein kinase B (Akt)/ mammalian target of rapamycin (mTOR) signaling pathway and effective mechanism of Yiqi Huoxue Fang depending on model of myocardial ischemia.Methods The model of acute myocardial infarction (AMI) was established through ligation of left coronary anterior descending branch in SD rats, and randomly divided into model group, perindopril group, Qishen Yiqi Gutta Pills group, Yiqi Huoxue Fang group and sham-operation group, and all groups were given orally corresponding medications respectively 2 d after operation.The observation time points were set on the 7th d and 28th d.The changes of heart function and structure were detected by using ultrasonic testing, changes of myocardial pathomorphology were observed after HE staining, and expressions of Akt, mTOR, p-Akt and p-mTOR in infarction marginal zone were detected by using Western blot test, real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry technique in all groups.Results The level of left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) decreased significantly in model group compared with sham-operation group on the 7th d and 28th d (P<0.01).The level of left ventricular end-systolic volume (LVESV) increased significantly on the 7th d (P<0.01), and levels of LVESV and left ventricular end-diastolic volume (LVEDV) increased significantly (P<0.01) on the 28th d in model group.The levels of LVEF and LVFS increased and levels of LVEDV and LVESV decreased significantly in all medicated groups compared with model group (P<0.01).The thinner ventricular wall, less cardiomyocytes, disorder myocardial cells arrangement and inflammatory cell infiltration were observed in infarction marginal zone in model group, and disorder myocardial cells arrangement and less cardiomyocytes were observed in all medicated groups.The ratio of p-Akt/Akt and p-mTOR/mTOR increased (P<0.05), expressions of Akt m-RNA and mTOR m-RNA increased significantly (P<0.01), and levels of p-Akt and p-mTOR positive cell rates increased significantly (P<0.01) in model group.The ratio of p-Akt/Akt and p-mTOR/mTOR increased (P<0.05), expressions of Akt m-RNA and mTOR m-RNA increased (P<0.05 or P<0.01), and levels of p-Akt and p-mTOR positive cell rates increased significantly (P<0.05) in all medicated groups.Conclusion Yiqi Huoxue Fang can improve heart function and protect myocardial cells through activating cardiac self-protective mechanism-Akt/mTOR signaling pathway in rats with myocardial infarction.关键词
急性心肌梗死/益气活血方/蛋白激酶B/雷帕霉素靶蛋白信号通路/大鼠Key words
acute myocardial infarction/Yiqi Huoxue Fang/Akt/mTOR signaling pathway/rats分类
医药卫生引用本文复制引用
刘文臣,谭晓波,蔡倩,郭书文,郑乘龙,王惠,张璐,吴佳妮,刘俊丽,黄小楼,李芳赫..益气活血方对心肌缺血大鼠Akt/mTOR信号通路的影响[J].北京中医药大学学报,2017,40(5):376-384,9.基金项目
国家自然科学基金项目(No.81473552,No.81173142)Project sponsored by National Natural Science Foundation (No.81473552, No.81173142) （No.81473552,No.81173142）
