北京中医药大学学报2017，Vol.40Issue(5)：392-398,7.DOI:10.3969/j.issn.1006-2157.2017.05.007
左归降糖解郁方对糖尿病并发抑郁症海马神经元CaMKⅡ及SynGap的调控作用
Regulating effect of Zuogui Jiangtang Jieyu Fang on hippocampus neuron synaptic plasticity related proteins-CaMKⅡ and SynGap in simulated condition of diabetes complicating depression
摘要
Abstract
Objective To investigate the influence of Zuogui Jiangtang Jieyu Fang (ZJJF) on hippocampus neuron synaptic plasticity related proteins-CaMKⅡ and SynGap in rats simulated diabetes complicating depression DD.Methods The hippocampus neurons were primitively isolated, purified and cultured in SD rats.The DD model was established by applying intervention of high glucose and cortisone.The cultured hippocampus neurons were randomly divided into normal group, model group, medicated serum of ZJJF (ZJJF group), medicated serum of positive drug (fluoxetine and metform) group (positive drug group) and blank group.The damages of hippocampus neurons were detected by using Nissl's staining in all groups, and expressions of CaMKII and SynGap were detected by using immunofluorescence staining and high-content analysis (HCA) after drug intervervention for 18 d.Results Compared with normal group and blank group, the quantity of Nissl bodies decreased significantly, neural network and dentritic spines ruptured or disappeared, and expressions of CaMKII and SynGap were significantly down-regulated in model group (P<0.01).Compared with model group, the quantity of Nissl bodies, neural network and dentritic spines, and synaptic connection were significantly recovered, and expressions of CaMKII and SynGap were un-regulated in positive drug group and ZJJF group (P<0.01 or P<0.05).Conclusion ZJJF had significant regulative effect on CaMKII and SynGap in simulated condition of diabetes complicating depression, which may be one of important mechanisms of ZJJF antagonizing cellular damage and neural degeneration.关键词
糖尿病并发抑郁症/细胞培养/海马神经元/左归降糖解郁方/钙调蛋白依赖性蛋白激酶Ⅱ/磷酸化认知缺陷突触相关蛋白/大鼠Key words
diabetes complicating depression/cell culture/hippocampus neurons/Zuogui Jiangtang Jieyu Fang/calmodulin dependent protein kinase II/SynGap/rats分类
医药卫生引用本文复制引用
刘检,王宇红,韩远山,徐雅岚,刘林,杨蕙,李薇,向韵..左归降糖解郁方对糖尿病并发抑郁症海马神经元CaMKⅡ及SynGap的调控作用[J].北京中医药大学学报,2017,40(5):392-398,7.基金项目
国家自然科学基金项目 (No.81373578, No.81573965),国家自然科学基金青年基金项目(No.81403379, No.81603604),湖南省自然科学基金项目(No.2017JJ3241)Project sponsored by National Natural Science Foundation (No.81373578, No.81573965), Youth Fund Project sponsored by National Natural Science Foundation (No.81403379, No.81603604), Natural Science Foundation of Hunan Province (No.2017JJ3241) （No.81373578, No.81573965）
