北京中医药大学学报2017，Vol.40Issue(5)：399-404,6.DOI:10.3969/j.issn.1006-2157.2017.05.008
蒙药嘎日迪散对溃疡性结肠炎大鼠Th1细胞和Th17细胞的影响
Influence of Mongolia medicine-Garidi San on Th1 and Th17 in rats with ulcerative colitis
摘要
Abstract
Objective To discuss the influence of Mongolia medicine-Garidi San on helper T cell 1 (Th1) and helper T cell 17 (Th17) in rats with ulcerative colitis (UC).Methods All rats were randomly divided into normal group, model group, low-dose Garidi San group (low-dose group), mid-dose Garidi San group (mid-dose group), high-dose Garidi San group (high-dose group), sulfasalazine group (SSZ group) and Bupi Yichang Wan group.The UC model was induced and established by using immune method and TNBS.Garidi San was orally given to, respectively, low-dose group (1.76 g/kg), mid-dose group (3.51 g/kg) and high-dose group (7.02 g/kg), SSZ was given to SSZ group (3.60 g/kg), and Bupi Yichang Wan was given to Bupi Yichang Wan group (1.62 g/kg).After 4 weeks the samples of peripheral blood were collected from all groups.The ratio of Th1 to Th17 (Th1/Th17) in peripheral blood was observed by using flow cytometry, and levels of PB transforming growth factor β (TGF-β), interleukin-6 (IL-6), interleukin-17 (IL-17) and interferon-γ (IFN-γ) were detected by using enzyme-linked immunosorbent assay (ELISA).Results The level of TGF-β was decreased significantly in mid-dose group, low-dose group and SSZ group.The levels of serum IL-6 and IL-17 were decreased significantly(P<0.05), level of IFN-γ was increased (P<0.05), and Th1/Th17 was increased (P<0.05) in all medication groups.Conclusion Garidi San can regulate the differentiation direction of helper T cell 0, and recover the balance of Th1/Th17 in UC rats, which may be one of mechanisms of Garidi San curing UC.关键词
嘎日迪散/溃疡性结肠炎/Th1细胞/Th17细胞/大鼠Key words
Garidi San/ulcerative colitis/helper T cell 1/helper T cell 17/rats分类
医药卫生引用本文复制引用
王琳,裴玲燕,柯愈诗,崔箭,李树春..蒙药嘎日迪散对溃疡性结肠炎大鼠Th1细胞和Th17细胞的影响[J].北京中医药大学学报,2017,40(5):399-404,6.基金项目
国家自然科学基金项目(No.81273872)Project sponsored by National Natural Science Foundation (No.81273872) （No.81273872）
