北京中医药大学学报2017，Vol.40Issue(5)：405-412,8.DOI:10.3969/j.issn.1006-2157.2017.05.009
黄芪甲苷通过激活自噬抑制缺氧缺糖/复氧复糖诱导的PC12细胞凋亡
Inhibitory effect of astragaloside IV on PC12 apoptosis induced by oxygen-glucose deprivation/oxygen-glucose rehabilitation through activating autophagy
摘要
Abstract
Objective To study the inhibitory effect of astragaloside IV (AST) on the apoptosis of PC12 cells induced by oxygen-glucose deprivation/oxygen-glucose rehabilitation (OGD/OGR) through activating autophagy.Methods PC12 cells in logarithmic phase were collected and randomly divided into normal group, OGD/OGR group (model group), AST solvent control group (DMSO group), AST group, AST+autophagy inhibitor group (AST+3-MA group), 3-MA group and autophagy activator group (RAPA group).Except of normal group, all other groups were given treating of OGD for 3 h and then OGR for 12 h and simultaneous medication treating.The morphological changes of autophagosomes were observed by using transmission electron microscope (TEM), expressions of LC3II, Beclin1 and Caspase-3 were detected by using Western blotting, and apoptosis of PC12 cells were detected by using flow cytometry after TUNEL staining.Results Compared with normal group, the typical autophagosomes were observed, expressions of LC3II, Beclin1 and Caspase3 increased, and apoptosis rate and apoptosis increased (P<0.05).Compared with model group, the quantity of autophagosomes and expressions of LC3II and Beclin1 increased (P<0.05), and expression of Caspase3 and apoptosis rate and apoptosis decreased in AST group and RAPA group (P<0.05).Compared with model group, the quantity of autophagosomes and expressions of LC3II and Beclin1 decreased (P<0.05), and expression of Caspase3 and apoptosis rate and apoptosis increased in 3-MA group (P<0.05), while DMSO group and model group had no significant difference (P>0.05).Compared with AST group, the quantity of autophagosomes and expressions of LC3II and Beclin1 decreased (P<0.05), and expression of Caspase3 and apoptosis rate and apoptosis increased in AST+3-MA group and 3-MA group (P<0.05).Conclusion AST play a neuroprotective role through activating autophagy and inhibiting apoptosis of PC12 cells induced by OGD/OGR.关键词
黄芪甲苷/细胞自噬/细胞凋亡/PC12细胞/缺氧缺糖/复氧复糖Key words
astragaloside/autophagy/apoptosis/PC12 cells/oxgen-glucose deprivation/oxgen-glucose rehabilitation分类
医药卫生引用本文复制引用
靳晓飞,周晓红,武密山,张颖,赵艳萌,李媛,吴增,高维娟..黄芪甲苷通过激活自噬抑制缺氧缺糖/复氧复糖诱导的PC12细胞凋亡[J].北京中医药大学学报,2017,40(5):405-412,8.基金项目
河北省应用基础研究计划重点项目(No.16967756D),河北省普通高校高层次人才科学研究计划项目(No.GCC2014031),河北省博士学位点科研能力建设项目(No.169677128D),河北省研究生创新资助项目(No.冀学位[2016]3号)Planned Key project of Applied Basic Research in Hebei Province (No.16967756D), Planned Project of High-level Talents in Ordinary Universities in Hebei Province (No.GCC2014031), Project of Construction of Capacity for Scientific Research in Doctor’S Degree Authorization Centers in Hebei Province (No.169677128D), Project of Supporting graduate Innovation in Hebei Province (No.Hebei academic degree[2016]3) （No.16967756D）
