北京中医药大学学报2017，Vol.40Issue(5)：413-419,7.DOI:10.3969/j.issn.1006-2157.2017.05.010
宁心安神方调控失眠大鼠Glu/GABA-Gln代谢环路失衡的机制研究
Mechanism of Ningxin Anshen Fang in regulation of unbalanced Glu/GABA-Gln metabolic loop in insomnia rats
摘要
Abstract
Objective To study the therapeutic effect of Ningxin Anshen Fang on insomnia, and regulative mechanism to the metabolic loop of glutamic acid (Glu)/γ-aminobutyric acid (GABA)-glutamine (Gln).Methods The model of insomnia was established by using modified multiple water platform sleep deprivation method in rats, and then all rats were randomly divided into normal group, model group, diazepam group, Ningxin Anshen Fang low-dose group (low-dose group), Ningxin Anshen Fang mid-dose group (mid-dose group) and Ningxin Anshen Fang high-dose group (high-dose group).The general conditions were observed, sleep latency and sleep duration were observed by applying pentobarbital sodium (PS) cooperative sleep test, and model and curative effect of Ningxin Anshen Fang on insomnia were reviewed.The content of Glu and GABA in brainstem and hypothalamus was detected by using high performance liquid chromatography (HPLC).The expressions of glutamic acid decarboxylase (GAD) and astrocyte glutamine synthetase (GS) in brainstem and hypothalamus were detected by using Western blot experiment.Results The sleep latency was significantly shortened, sleep duration was prolonged, GABA level in brainstem and hypothalamus was increased and ratio of Glu to GABA (Glu/GABA) was decreased by Ningxin Anshen Fang in insomnia rats.The expression of GAD in brainstem and hypothalamus was significantly promoted in all Ningxin Anshen Fang groups.Conclusion Ningxin Anshen Fang can affect the synthesis and decomposition Glu and GABA and expressions of Glu and GABA, and normalize the excitotary/inhibitory metabolic imbalance of Glu/GABA through regulating Glu/GABA-Gln metabolic loop, which may be related to that Ningxin Anshen Fang has the curative effects of calming heart and tranquillizing mind, and sedation and hypnosis.关键词
谷氨酸/γ-氨基丁酸/宁心安神/失眠/大鼠Key words
glutamic acid/γ-aminobutyric acid/calming heart and tranquillizing mind/insomnia/rats分类
医药卫生引用本文复制引用
郭晓,郭蓉娟,邢佳,王嘉麟,袁清洁,王建伟,高维..宁心安神方调控失眠大鼠Glu/GABA-Gln代谢环路失衡的机制研究[J].北京中医药大学学报,2017,40(5):413-419,7.基金项目
北京市科技计划课题项目(No.Z161100001816010),北京中医药大学东方医院人才培养计划Project sponsored by Beijing Science and Technology Project(No.Z161100001816010) （No.Z161100001816010）
