中国耳鼻咽喉头颈外科2017，Vol.24Issue(5)：253-257,5.DOI:10.16066/j.1672-7002.2017.05.011
免疫耐受型树突状细胞对变应性鼻炎小鼠的调控作用
Immunoregulatory role of tolerogenic dendritic cells in allergic rhinitis in mice
摘要
Abstract
OBJECTIVE To investigate the immunoregulatory role of tolerogenic dendritic cells(TolDC) in allergic airway inflammation in mouse model of allergic rhinitis(AR).METHODS A total of 24 Balb/c mice were equally and randomly divided into 4 groups with 6 mice in each group. AR group was established with oval bumin(OVA) sensitization and challenge, meanwhile the physiological saline(PBS) sensitization and challenge as the control group. AR group were treated by adoptive transfer of TolDC as treatment group. And treatment group were injected intraperitoneally with TGF-β/IL-10R neutralizing antibody as blockade group. In each group, allergic nasal symptoms score, inflammatory cell infiltration in lung, the expression of Th1/Th2-derived cytokines in the bronchoalveolar lavage fluid(BALF) and nasal lavage fluid(NALF), the expression of OVA-specific serum IgE and the expression of Treg in lung were measured.RESULTS The mouse model of OVA-induced AR was successfully developed. Compared with AR group, treatment group exhibited lower allergic nasal symptoms score, a decrease in inflammatory cell infiltration in lung, lower expression of Th1/Th2-derived cytokines in BALF and NALF and OVA-specific serum IgE, as well as up-regulation of Treg in lung, which were abolished by TGF-β/IL-10 neutralizing antibody shown in blockade group.CONCLUSION TolDC suppress airway inflammation in AR by inducing regulatory T cells through TGF-β/IL-10-dependent mechanisms.关键词
鼻炎,变应性,常年性/鼻炎,变应性,季节性/树突细胞/免疫耐受/T淋巴细胞/免疫调节/免疫耐受型树突状细胞/调节性T细胞Key words
Rhinitis/Allergic/Perennial/Rhinitis/Allergic/Seasonal/Dendritic Cells/Immune Tolerance/T-Lymphocytes/Immunomodulation/tolerogenic dendritic cells/regulatory T cell引用本文复制引用
魏佳炜,钟华,李航,文卫平..免疫耐受型树突状细胞对变应性鼻炎小鼠的调控作用[J].中国耳鼻咽喉头颈外科,2017,24(5):253-257,5.基金项目
国家自然基金(81271055、81470674、81670902),科 技部"十二五"国家科 技 支 撑计划课题(2014BAI07B04)以及广东省自然基金(2016A030310153)联合资助 （81271055、81470674、81670902）
