高等学校化学学报2017，Vol.38Issue(5)：738-742,5.DOI:10.7503/cjcu20170105
牛油果中化学成分的内部萃取电喷雾电离质谱检测
Direct and Rapid Analysis of Avocado Using Internal Extractive Electrospray Ionization Mass Spectrometry
摘要
Abstract
Avocado plays significant roles in food and medicine industries, due to its advantages of high nutrition profile, low sugar and high energy.In this work, the chemical compositions of pulp and peel of avocado were fast analyzed by internal extraction electrospray ionization mass spectrometry technology(iEESI-MS) without any pretreatment.Furthermore, Orthogonal partial least squares discriminant analysis(OPLS-DA) model was established to distinguish avocado's maturity degree between unripening and ripening.The experimental results indicated that in negative ion mode, a total of 12 compounds such as palmitic acid, linolenic acid, palmitic acid, linolenic acid, oleic acid, stearic acid in pulp of avocado were indentified;while 11 compounds in peel of avocado were indentified including cianidanol, chlorogenic acid, pantothenic acid and so on.The differences of chemical compounds between unripening and ripening could be obviously observed through the model of OPLS-DA.The merits of this method to analyze compounds of avocado by iEESI-MS include simplicity, excellent specificity, high throughput and minimum sample pretreatment.关键词
牛油果/内部萃取电喷雾电离质谱/化学成分/成熟程度Key words
Avocado/Internal extraction electrospray ionization mass spectrometry/Composition/Maturity分类
化学化工引用本文复制引用
柯牡芳,韩京,朱腾高,刘文杰,张华,寇伟,梁大鹏..牛油果中化学成分的内部萃取电喷雾电离质谱检测[J].高等学校化学学报,2017,38(5):738-742,5.基金项目
国家自然科学基金(批准号:21307040,21427802)和江西省质谱科学与仪器重点实验室开放基金(批准号:JXMS201601)资助.Supported by the National Natural Science Foundation of China(Nos.21307040,21427802) and the Open Found of Jiangxi Provincial Key Laboratory for Mass Spectrometry and Instrumentation,China(No.JXMS201601). （批准号:21307040,21427802）
