口腔疾病防治2017，Vol.25Issue(5)：294-299,6.DOI:10.12016/j.issn.2096-1456.2017.05.004
自噬相关蛋白PTEN、MAP1LC3在舌癌中的表达与意义
Expression of autophagy-related proteins PTEN and MAP1LC3 in tongue squamous cell carcinoma
摘要
Abstract
Objective This study aims to evaluate the expression of autophagy-related protein cancer phosphatase and tensinhomology deleted on chromosome ten (PTEN) and microtubule-associated protein 1 light chain 3 (MAP1LC3) and investigate its significance in tongue squamous cell carcinoma (TSCC). Methods About 46 TSCC samples, 13 cas-es of precancerous lesion and 13 cases of adjacent tissues were enrolled. Immunohistochemistry were performed to ex-amine the expression of PTEN, MAP1LC3. Statistical analyses were carried out to assess the associations among clinic pathologic parameters. Results The positive expression rate of PTEN (34.78%) in TSCC was significantly lower than that in adjacent tissues (69.23%, X2=4.926, P=0.026) and precancerous lesions (76.92%, X2=7.302, P=0.007). The positive expression rate of MAP1LC3 (28.26%) in TSCC was significantly lower than that of adjacent tissues (61.53%,X2=4.896, P=0.027) and precancerous lesions (69.23%, X2=7.275, P=0.007), the difference was statistically signifi-cant. The expression of PTEN and MAP1LC3 was not correlated with age, sex, smoking, alcohol consumption and cell differentiation (P>0.05). There was a significant difference in the expression of PTEN and MAP1LC3 in subgroup with different clinical stage and in subgroup with or without lymph node metastasis (P < 0.05). There is a significant differ-ence in the tumor recurrence between PTEN protein expression positive subgroup and PTEN protein expression nega-tive subgroup (X2 = 4.629,P = 0.039), and there was no significant difference in the tumor recurrence between MAP1LC3 protein expression positive subgroup and MAP1LC3 protein expression negative subgroup (X2 = 0.343,P =0.453). There was a positive correlation between PTEN and MAP1LC3. Conclusion The expression of PTEN and MAP1LC3 suggested that autophagy associated proteins play a pivotal role in the progression, diagnosis and prognosis of TSCC. Down-regulation of PTEN and MAP1LC3 was observed in TSCC.关键词
舌鳞状细胞癌/自噬/PTEN/MAP1LC3/预后Key words
Tongue squamous cell carcinoma/Autophagy/PTEN/MAP1LC3/Prognosis分类
医药卫生引用本文复制引用
王亚楠,刘浩,张军..自噬相关蛋白PTEN、MAP1LC3在舌癌中的表达与意义[J].口腔疾病防治,2017,25(5):294-299,6.基金项目
天津市卫计委科技基金项目(2013KZ053) （2013KZ053）
