影像科学与光化学2017，Vol.35Issue(3)：265-273,9.DOI:10.7517/j.issn.1674-0475.2017.03.265
基于罗丹明和BODIPY单元的FRET探针的合成及其对小分子α-酮戊二酸的检测
A FRET Fluorescent Probe for α-Ketoglutaric Acid Based on Rhodamine and BODIPY
摘要
Abstract
Small molecule α-ketoglutaric acid (α-KA) is a very important product of metabolism in human body,which exhibits direct or indirect relation with many kinds of diseases.A fluorescence resonance energy transfer (FRET) ratiometric probe for α-KA containging rhodamine and BODIPY has been synthesized.As the energy donor,the fluorescence spectrum of BODIPY matches well with the absorption spectrum of rhodamine which is chosen as the energy acceptor.Research shows that the orginal fluorescence spectrum of probe RDM-BO is similar with BODIPY.While the α-KA induces ring-open reacntion with rhodamine unit of RDM-BO,the molecular conjugate is changed,resulting in new fluorescence peaks.The detection limit could reach 3.14 tmol · L-1.关键词
罗丹明/BODIPY/α-酮戊二酸/荧光探针Key words
rhodamine/BODIPY/α-ketoglutaric acid/fluroescence probe引用本文复制引用
何业,唐俊马,郭志前,靳鹏伟,朱世琴,朱为宏..基于罗丹明和BODIPY单元的FRET探针的合成及其对小分子α-酮戊二酸的检测[J].影像科学与光化学,2017,35(3):265-273,9.基金项目
国家973计划(2013CB733700)和国家自然科学基金(21476076)资助 （2013CB733700）
