广西医科大学学报2017，Vol.34Issue(5)：654-658,5.DOI:10.16190/j.cnki.45-1211/r.2017.05.004
钛离子对Jurkat T细胞增殖及其胞内钙离子浓度的影响
Effect of titanium ions on the proliferation and intracellular calcium concentration in Jurkat T cells
摘要
Abstract
Objective:To investigate the effect of titanium ions on the changes of intracellular calcium concentration and proliferation in Jurkat T cells.Methods:MTT assay was performed to determine the concentrations of titanium ions at which the cell proliferation rate was increased by 50％ or 95％.The Jurkat T cells were divided into two groups:T cells pre-activated by phytohemagglutinin (PHA) or resting T cells without pre-activation.After co-culture with titanium ions at selected concentration for 24 hours,the distribution of calcium cations and content changes were detected by the laser scanning confocal microscope.Results:(1) When the concentration of titanium ions was lower than 100 μmol/L,T cells could be activated and the proliferate rate was increased along with the concentration of titanium ions.When the concentration of titanium was between 100-200 μmol/L,the proliferation rate declined.Once the concentration of titanium ions exceeded 200 μmol/L,cells proliferation were suppressed.(2) When extracellular solution containing Ca2+,after treating with different concentrations of titanium ions (5 μmol/L,10 μmol/L,50 μmol/L and 100 μmol/L),the intracellular calcium concentration increased in both resting cells group and activated cells group,and the rise within activated T cells group was much more significant (P ＜ 0.05).Compared to non treated control cells,the difference were significant (P ＜0.05).When extracellular solution was without Ca2+,the intracellular Ca2+ concentration was not significantly changed in both groups,and compared with the control group,the difference was not statistically significant as well (P ＞ 0.05).Conclusion:Concentration of titanium ions below 100μmol/L could promote Jurkat T cells' activation and proliferation.Titanium ions could stimulate the pre-actived T cells when extracellular solution containing Ca2+,which resulted in the increase of intracellular calcium concentration.关键词
钛离子/T细胞/Ca2+Key words
titanium ions/T-lymphocytes/calcium ion分类
医药卫生引用本文复制引用
李昌,李秋莹,杨倩,陈富军,唐礼..钛离子对Jurkat T细胞增殖及其胞内钙离子浓度的影响[J].广西医科大学学报,2017,34(5):654-658,5.基金项目
国家自然科学基金资助项目(No.81160136) （No.81160136）
