广西医科大学学报2017，Vol.34Issue(5)：659-662,4.DOI:10.16190/j.cnki.45-1211/r.2017.05.005
缺血后调适对大鼠骨胳肌缺血再灌注损伤的作用及其机制研究
The effect and mechanism of ischemic post-conditioning on skeletal muscle ischemia-reperfusion injury in rats
摘要
Abstract
Objective:To investigate the effect and mechanism of ischemic post-conditioning (IPoC) on skeletal muscle ischemia-reperfusion (SMIR) injury in rats.Methods:18 rats were randomly divided into 3 groups (n =6 in each group):sham group,SMIR group and IPoC group.The rats in the SMIR group were subjected to 4 hours ischemia of the right hind limb,followed by reperfusion.Rats in IPoC group were subjected to right hind limb occasion for 4 hours,followed by four cycles of 60-s intervals of ischemia and reperfusion starting at 30 s after the initial reperfusion.The expressions of RISK signaling pathway related proteins such as Akt,Erk1/2,phosphorylated (p) Akt and p-Erk1/2 were detected by western blotting.The opening of mitochondrial permeability transition pore (MPTP) was observed by UV spectrophotometer.The ratio of wet/dry weight (W/D) of gastrocnemius was measured after 24 h.Results:(1) IPoC could maintained the acidic environment in the tissues within 2.67 min,and the pH value was (6.81± 0.133) (P ＜0.01).(2) The ratios of p-Akt/t-Akt and p-Erk1/2/t-Erk1/2 in IPoC group were increased,while were decreased in the SMIR group compared with the sham group (P ＜0.01).(3) At 2 and 4 min,the opening of MPTP in IPoC group was increased than that in the other two groups (P ＜0.05).(4) The W/D ratio of IPoC group was significantly lower than that of SMIR group (P ＜0.05).but there was no significant difference between sham group and IPoC group (P ＞0.05).Conclusion:IPoC could effectively alleviate SMIR injury in rats,and the mechanisms might be related to maintaining acid environment in initial reperfusion and activating the RISK signaling pathway.关键词
骨骼肌/缺血再灌注损伤/缺血后调适/pH值/RISK通路Key words
skeletal muscle/ischemia-reperfusion injury/ischemic post-conditioning/pH/RISK pathway分类
医药卫生引用本文复制引用
阮思杰,阳富春,阳茂春,刘军廷,胡峰,王静威..缺血后调适对大鼠骨胳肌缺血再灌注损伤的作用及其机制研究[J].广西医科大学学报,2017,34(5):659-662,4.基金项目
国家自然科学基金资助项目(No.81260276) （No.81260276）
