华南农业大学学报2017，Vol.38Issue(3)：46-51,6.DOI:10.7671/j.issn.1001-411X.2017.03.008
2株枯草芽孢杆菌的分离鉴定及特性研究
Isolation, identification and characterization of two strains of Bacillus subtilis
摘要
Abstract
[Objective] To explore potential probiotics in order to develop microecological agents for aquiculture.[Method]Bacillus stains were isolated from the pond sediment and healthy carp (Carassius auratus) intestine.The strains were identified by the morphological, physiological and biochemical characteristics and 16S rRNA sequence analysis.The safety, high temperature tolerance, pH tolerance, antagonistic effects and enzyme production of the strains were studied.[Result] Two Bacillus strains were isolated and named B1 and B2.They were identified as B.subtilis by morphology, physiological and biochemical characteristics and 16S rRNA sequence analysis.The B1 and B2 strains had good safety, high temperature tolerance and strong tolerance of acid.They had good antibacterial activity against pathogenic Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila and A.sobria in vitro, and had the ability to produce lipase, amylase, protease and cellulase.[Conclusion]Two B.subtilis strains were isolated, and both can be used as candidates for developing microecological agents for aquiculture.关键词
枯草芽孢杆菌/分离鉴定/菌株特性/鲫鱼/16SrRNAKey words
Bacillus subtilis/isolation and identification/strain characteristic/Carassius auratus/16S rRNA分类
农业科技引用本文复制引用
巴翠玉,张林波,张培军,赵福广,李月红..2株枯草芽孢杆菌的分离鉴定及特性研究[J].华南农业大学学报,2017,38(3):46-51,6.基金项目
国家自然科学基金(30972191) （30972191）
吉林省现代农业产业技术体系(201506),吉林省留学人员创新创业项目(201523) （201506）
