华南农业大学学报2017，Vol.38Issue(3)：52-56,5.DOI:10.7671/j.issn.1001-411X.2017.03.009
香蕉枯萎病菌4个生理小种核糖体DNA内转录间隔区的RFLP分析
RFLP analysis of the ribosomal DNA ITS regions of four physiological races of Fusarium oxysporum f.sp.cubense
摘要
Abstract
[Objective] To clarify the phylogenetic relationship of four physiological races of Fusarium oxysporum f.sp.cubense (Foc), the causal agent of fusarial wilt of banana.[Method] The ribosomal DNA internal transcribed spacer (ITS) regions of the four physiological races of Foc were first amplified using PCR technique, and then RFLP analysis of the ITS products of these races were performed using three restriction endonucleases including AIu I, Hpa II and Taq I.[Result]A single 568 bp ITS fragment was amplified specifically from eight strains belonging to four races, respectively, but there was no obvious polymorphism among these races.RFLP analysis of the ITS products from these races showed that the differences among races were very small.[Conclusion] PCR-RFLP technique was unsuitable to be used as the identification basis for the physiological races of Foc.关键词
香蕉枯萎病菌/生理小种/内部转录间隔区/聚合酶链式反应/限制性片段长度多态性Key words
Fusarium oxysporum f.sp.cubense/physiological race/internal transcribed spacer (ITS)/polymerase chain reaction (PCR)/restriction fragment length polymorphism (RFLP)分类
农业科技引用本文复制引用
舒灿伟,曾蕊,杨媚,周而勋..香蕉枯萎病菌4个生理小种核糖体DNA内转录间隔区的RFLP分析[J].华南农业大学学报,2017,38(3):52-56,5.基金项目
国家现代农业(香蕉)产业技术体系建设专项(CARS-32) （香蕉）
