华西口腔医学杂志2017，Vol.35Issue(3)：275-280,6.DOI:10.7518/hxkq.2017.03.009
柚皮苷协同骨形态发生蛋白-2促进小鼠成骨细胞MC3T3-E1增殖和分化的研究
Effect of naringin combined with bone morphogenetic protein-2 on the proliferation and differentiation of MC3T3-E1 cells
摘要
Abstract
ObjectiveThis study evaluates the biological effects of naringin (NAR) joint bone morphogenetic protein (BMP)-2 on the proliferation, alkaline phosphatase (ALP) activity, and expression of osteoblastogenic genes, such as Runt-related transcription factor 2 (Runx2), collagenⅠ (ColⅠ), ALP, and osteocalcin (OCN) of pre-osteoblasts.Methods Three different NAR concentrations (10, 100, and 1000 μmol·L-1) were applied, alone or combined with BMP-2(50 ng·mL-1), to restore the osteoblastogenesis of pre-osteoblasts (MC3T3-E1 cell line). Cell numbers (proliferation) were evaluated at first, fourth, and seventh days by Alamar blue assay. ALP activity and the expression of osteoblastogenic genes, such as Runx2, ColⅠ, ALP, and OCN were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) at fourth and seventh day.Results Stimulation by NAR alone and in combination with BMP-2 for 1 day and 4 days could promote cell proliferation, which peaked at a concentration of 100 μmol·L-1 NAR combined with BMP-2 could promote cell proliferation significantly (P<0.05). Stimulation by NAR alone and in combination with BMP-2 for 4 and 7 days could promote ALP activity and bone-related gene(ALP, OCN, Runx2, ColⅠ) expression. ALP expression was significantly promoted after stimulation of 100 μmol·L-1 NAR and BMP-2 (P<0.05).Conclusion NAR exhibits promising potential for improving MC3T3-E1 proliferation and differentiation, and appropriate concentrations of NAR and BMP-2 show synergistic effect.关键词
成骨细胞/柚皮苷/骨形态发生蛋白-2/增殖/分化Key words
osteoblast/naringin/bone morphogenetic protein-2/proliferation/differentiation分类
生物科学引用本文复制引用
徐高丽,柳毅,吴立立,史秋涛,霍光,谷志远..柚皮苷协同骨形态发生蛋白-2促进小鼠成骨细胞MC3T3-E1增殖和分化的研究[J].华西口腔医学杂志,2017,35(3):275-280,6.基金项目
浙江省中医药科技计划项目(2014ZB028) （2014ZB028）
