海洋科学2017，Vol.41Issue(2)：53-60,8.DOI:10.11759//hykx20160113004
恩诺沙星对脊尾白虾APND、ECOD和GST活性的影响
Effects of enrofloxacin on APND, ECOD and GST activities in Exopalaemon carinicauda
摘要
Abstract
To study the response of drug metabolism enzymes to enrofloxacin and their function in the metabolism of enrofloxacin, shrimp (Exopalaemon carinicauda) were fed with medicated feed at doses of 10, 20, and 40 mg/(kg·BW) daily, and shrimp in each group were sampled on day 1 and day 2 during the drug exposure period and 1, 4, 8, 12, 24, 48, 72, and 120 h after the end of the drug exposure period. Levels of aminopyrine-N-demethylase (APND), 7-ethoxycoumarin-O-deethylase (ECOD), and glutathione-S-transferase (GST) were determined in the hepatopancreas, gills, and serum. Results showed that different concentrations of enrofloxacin could significantly inhibit the activi-ties of phase I metabolism enzymes APND and ECOD in the hepatopancreas, gills, and serum (P<0.05). After feeding with the drug, the activities of APND and ECOD first decreased and then increased gradually. Activities of the metabolism enzymes in the three concentration groups were lowest at 8 h after dosing. Compared with the con-trol group, different concentrations of enrofloxacin significantly induced the activities of phase Ⅱ metabolism en-zyme GST in the three tissues (P<0.05), and the induction of the activities were first increased and then decreased gradually. The effects of enrofloxacin on the activities of APND, ECOD, and GST were all dose dependent. In con-clusion, this study demonstrated that phase Ⅰ and phase Ⅱ enzymes of E. carinicauda are sensitive parameters that could be used as biomarkers for evaluating the exposure to aquatic drugs.关键词
脊尾白虾(Exopalaemoncarinicauda)/恩诺沙星/氨基比林-N-脱甲基酶(APND)/7-乙氧基香豆素-O-脱乙基酶(ECOD)/谷胱甘肽-S-转移酶(GST)Key words
Exopalaemon carinicauda/enrofloxacin/aminopyrine-N-demethylase (APND)/7-ethoxycoumarin-O-deethylase (ECOD)/glutathione-S-transferase (GST)分类
农业科技引用本文复制引用
翟倩倩,李健..恩诺沙星对脊尾白虾APND、ECOD和GST活性的影响[J].海洋科学,2017,41(2):53-60,8.基金项目
山东省自然科学基金项目(BS2015HZ015) （BS2015HZ015）
国家虾现代产业技术体系项目(CARS-47) （CARS-47）
山东泰山产业领军人才工程项目(LJNY2015002) （LJNY2015002）
青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02) Natural Science Foundation of Shandong Province, No.BS2015HZ015 （2015ASKJ02）
Modern Agro-industry Technology Research System, No.CARS-47 （）
the Program of Shandong Leading Talent No.LJNY2015002 （）
Ao Shan Scientific and Technological Innovation Project Financially Sup-ported by Qingdao National Laboratory for Marine Science and Technology, No.2015ASKJ02 （）
