海洋科学2017，Vol.41Issue(2)：71-80,10.DOI:10.11759/hykx20160811002
紫菜中类菌孢素氨基酸纯化工艺的优化及其抗紫外辐射作用研究
Optimization of purification process of mycosporine-like amino acid from Porphyra haitanensis and study on its anti-ultraviolet activity
摘要
Abstract
Objective: To explore the optimal techniques in the separation of mycosporine-like amino acids (MAAs) in Por-phyra haitanensis with cation-exchange resins and the study on its anti-ultraviolet activity after purification. Method: In the screening cation-exchange resin through the static adsorption and desorption analytical experiment, response-surface methodology was employed to establish a mathematical regression model with the dynamic ad-sorption and desorption in the single-factor experiments for SA-2. The ultraviolet-radiation models (UVA and UVB) when compared with blank samples showed that the survival rates of the MAAs groups of E. coli and S. aureus in-creased; this proved that the MAAs after purification had certain anti-ultraviolet activity. Result: SA-2 was found to be the most suitable resin for the mycosporine-like amino acid purification; the obtained adsorption and desorption rates were 86% and 56.63%, respectively. The optimum conditions for the purification were obtained when the sample containing 3 g/L MAAs at pH 6 was mounted onto a column at a flow rate of 1 mL/min; fur-ther, it was eluted with 3% ammonium hydroxide to pH 6 at a flow rate of 1.0 mL/min. The purification process yielded an adsorption and desorption rate of 79.21% and 64.02%, respectively. After the purification of MAAs sample, it had significant resistance to the ultraviolet radiation and the deactivation rate for E. coli and S. aureus decreased to 39.10% and 40.58%, respectively.关键词
坛紫菜/类菌孢素氨基酸/SA-2树脂/纯化/响应面/抗紫外辐射Key words
Porphyra haitanensis/mycosporine-like amino acids/SA-2 resin/purification/response surface/ultraviolet-resistant activity分类
轻工纺织引用本文复制引用
应锐,张朝辉,段筱杉,赵雪,侯虎,李八方..紫菜中类菌孢素氨基酸纯化工艺的优化及其抗紫外辐射作用研究[J].海洋科学,2017,41(2):71-80,10.基金项目
国家自然科学基金(31272705) （31272705）
海洋公益性行业科研专项(201505022-5) National Natural Science Foundation of China, No.31272705 （201505022-5）
Public Science and Technology Research Funds projects of ocean, No.201505022-5 （）
