海洋科学2017，Vol.41Issue(2)：103-110,8.DOI:10.11759//hykx20160319001
基于COI基因和D-loop区部分序列比较雷州半岛东、西岸湖栖鳍虾虎鱼群体的遗传多样性
Genetic diversity of Gobiopterus lacustris populations from western and eastern Leizhou Peninsula based on partial se-quences of mitochondrial COI gene and D-loop
摘要
Abstract
We compared two Gobiopterus lacustris populations collected from the eastern and western Leizhou Peninsula based on COI gene and D-loop region. The partial sequences of COI gene from 44 individuals (21 from eastern Jiulongshan population and 23 from western Gaoqiao population) and D-loop region from 53 individuals (25 from JLS and 28 from GQ) were cloned. Based on COI gene, the results were as follows: there were 29 variable sites in 619 bp COI sequences. The frequencies of T, C, A, and G were 27.8%, 31.0%, 23.7%, and 17.5%, respec-tively, with more A + T (51.5%) than C + G (48.5%). The haplotype diversity of Jiulongshan population (0.695) was higher than that of Gaoqiao population (0.324). Genetic differentiation analysis indicated that the eastern and west-ern populations were differentiated significantly (Snn = 0.591, 0.01 < P < 0.05). The correlative values of Nm, Gst, Fst, Kxy, and Dxy were 5.37, 0.147, 0.042, 2.547, and 0.004, respectively. However, much more variable sites (133 bp) were observed in D-loop regions with 563 bp length. In addition, there were higher frequencies of A + T (67.8%) than COI, and the haplotype diversity of Jiulongshan population (0.797) was higher than that of Gaoqiao population (0.661). Genetic differentiation of the eastern and western populations was highly significant (Snn = 0.970, P <0.001). The values of Nm, Gst, Fst, Kxy, and Dxy were 0.157, 0.315, 3.506, and 0.006, respectively. Analysis of molecular variance revealed that the percentage of variation within populations was higher than that among popula-tions. Cluster analysis showed the presence of clustering phenomenon, which gathered by the collected sites of sample based on COI gene and D-loop region, but D-loops were more apparent. In summary, a significant differen-tiation occurred between eastern and western Leizhou Peninsula.关键词
COI/D-loop/湖栖鳍虾虎鱼(Gobiopteruslacustris)/雷州半岛群体/遗传分化Key words
COI gene/D-loop region/Gobiopterus lacustris/population of Leizhou Peninsula/genetic differentiation分类
农业科技引用本文复制引用
廖健,龙水生,贺亮,郭昱嵩,刘楚吾,刘丽,王中铎..基于COI基因和D-loop区部分序列比较雷州半岛东、西岸湖栖鳍虾虎鱼群体的遗传多样性[J].海洋科学,2017,41(2):103-110,8.基金项目
国家自然科学基金项目(31201996) （31201996）
广东省高等学校优秀青年教师培养计划项目(Yq2013089) （Yq2013089）
广东海洋大学优秀学位论文培育项目(201605) National Natural Science Foundation of China, No.31201996 （201605）
Project of Outstanding Young Teacher Training Program in Colleges and Universities of Guangdong province, No. Yq2013089 （）
Excellent Thesis Breeding Program of Guangdong Ocean University, No. 201605 （）
