海洋科学2017，Vol.41Issue(2)：117-124,8.DOI:10.11759/hykx20160428001
混合菌群发酵马尾藻作为海藻肥料的研究
Studies on fermentation of Sargassum by mixed microorganism utilized as seaweed fertilizer
摘要
Abstract
Fermentative microorganisms present on the surface of seaweed were screened and then identified by 16S rDNA sequence analysis. We obtained the following four fermentative strains: Bacillus megaterium, Leclerciaadecarb-oxylata, Lysinibacillusmacroides, and Sphingomonasleidyi. Using controlled inoculation proportion of these four strains, we obtained a stable fermentation product. We studiedthe changes and mechanisms of seaweed fermentation processto provide theoretical and data support for industrialization production of seaweed fermentation fertilizer. At 36 h in the fermentation process, alginate oligosaccharides with polymerization degrees of 2–5 were generated and accompanied by secondary growth of fermentative strains and a decreased pH, which were the parameters that could be easily observed and monitored. Fermentation broth diluted 600 times could significantly promote the germination and root growth of wheat seeds. Germination rate was 26% higher and root growth was 37% longer than those in the control group, respectively. These results provided a feasible strategy for the production of seaweed fertilizer by microbial fermentation. This simple and effective fermentation method has strong operability and broad application prospects.关键词
发酵/马尾藻/褐藻寡糖/促生长Key words
fermentation/Sargassum/alginate oligosaccharide/growth promotion分类
生物科学引用本文复制引用
王明鹏,陈蕾,刘正一,王学江,秦松,闫培生..混合菌群发酵马尾藻作为海藻肥料的研究[J].海洋科学,2017,41(2):117-124,8.基金项目
威海市科技发展研究计划重点项目(2010-3-96) （2010-3-96）
国家"十二五"科技支撑计划项目(2013BAB01B0) （2013BAB01B0）
中国科学院科技服务网络计划项目(KFJ-EW-STS-060) （KFJ-EW-STS-060）
国家自然科学基金青年基金项目(41401285) （41401285）
河口与海岸学国家重点实验室开放课题项目(SKLEC-KF201412) （SKLEC-KF201412）
海洋公益性行业科研项目(201505022) Science and Technology Development Project of Weihai (2010-3-96), National Key Technology R&D Program of China (2013BAB01B0), Science and Technology Service Network Initiative of Chinese Academy of Sciences (KFJ-EW-STS-060), Young Scientists Fund of National Natural Science Foundation of China (41401285), Open Foundation of State Key Laboratory of Estuarine and Coastal Research (SKLEC-KF201412), PublicScience and Technology Research Funds Projects of Ocean (201505022) （201505022）
