解放军医学院学报2017，Vol.38Issue(5)：467-470,480,5.DOI:10.3969/j.issn.2095-5227.2017.05.022
miR-144-3p靶向调控跨膜蛋白16A基因抑制骨肉瘤143B细胞的增殖和侵袭实验
Effect of miR-144-3p on osteosarcoma 143B cells proliferation and invasion by regulating transmembrane protein 16 expression: An experimental study
摘要
Abstract
Objective To identify the expressions of miR-144-3p and transmembrane protein 16 (TMEM16A) in osteosarcoma 143B cells and explore the effect of miR-144-3p which may regulate TMEM16A expression on cell proliferation and invasion.Methods MiR-144-3p was predicted by bioinformatics which may regulate TMEM16A expression. After transfection of miR-144-3p mimics or siRNA against TMEM16A into cells, expressions of miR-144-3p and TMEM16A were detected by real-time PCR or western blot. The proliferation and invasion of 143B cells were respectively determined using MTT assay and transwell chamber assay. Results MiRanda and TargetScan showed that there was a good complementary relationship between miR-144-3p and TMEM16A gene. Results of real-time PCR and western blot showed over-expression of miR-144-3p downregulated the expression levels of TMEM16A mRNA and protein. The proliferation and invasion of 143B cells were suppressed after transfection of miR-144-3p mimics or siRNA against TMEM16A. Conclusion MiR-144-3p may inhibit TMEM16A expression in osteosarcoma 143B cells and subsequently suppress cell proliferation and invasion.关键词
骨肉瘤143B细胞/微小RNA/跨膜蛋白16AKey words
osteosarcoma 143B cells/miRNA/TMEM16A分类
医药卫生引用本文复制引用
王涛,田鹤,穆长征..miR-144-3p靶向调控跨膜蛋白16A基因抑制骨肉瘤143B细胞的增殖和侵袭实验[J].解放军医学院学报,2017,38(5):467-470,480,5.基金项目
辽宁省自然科学基金资助(2015020334) Supported by the Natural Science Foundation of Liaoning Province (2015020334) （2015020334）
