临床肝胆病杂志2017，Vol.33Issue(5)：875-879,5.DOI:10.3969/j.issn.1001-5256.2017.05.016
特异性下调LKB1的表达对肝癌细胞侵袭迁移能力的影响
Influence of specific down-regulation of LKB1 expression on invasion and migration of hepatoma cells
摘要
Abstract
Objective To investigate the influence of specific down-regulation of LKB1 expression on invasion and migration of the hepatoma cell line SK-hep-1.Methods Quantitative real-time PCR (RT-PCR) and Western blot were used to measure the expression of LKB1 in the human hepatoma cell line SK-hep-1 and normal hepatocytes (HL7702).LKB1 specific double-strand small interfering RNA was designed,synthesized,and then transfected into the human hepatoma cell line SK-hep-1 with high expression of LKB1 to silence the expression of LKB1 gene.A negative control (NC) group was established.RT-PCR and Western blot were used to measure the changes in the mRNA and protein expression of LKB1.Cell Counting Kit-8 was used to measure the proliferative capacity of hepatoma cells.In vitro Transwell chamber invasion and migration assays were used to observe the influence of LKB1 gene silencing on invasion and migration of the human hepatoma cell line SK-hep-1.The t-test was used for comparison of continuous data between two groups;a one -way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between any two groups.Results Compared with normal human hepatocytes (HL7702),SK-hep-1 cells had significantly higher mRNA and protein expression of LKB1 (both P ＜ 0.05).Compared with the NC group,the siLKB1-1 treatment group had significant reductions in the mRNA and protein expression of LKB1 in SK-hep-1 cells (both P ＜ 0.05).Compared with the NC group,the siLKB1-1 treatment group had a significantly higher cell proliferation rate and significant increases in invasion and migration (1.393 ± 0.022 vs 1.1284 ±0.032,t =15.313,P ＜0.001;147 ± 19 vs 83 ±21,t =14.879,P ＜0.001;113 ± 13 vs 75 ± 17,t =8.791,P ＜0.001).Conclusion LKB1 may be involved in inhibiting the proliferation,invasion,and migration of hepatoma cells and can affect the prognosis of patients with liver cancer.关键词
癌,肝细胞/LKB1/细胞增殖/肿瘤侵润/细胞运动Key words
carcinoma, hepatocellular/liver kinase b1/cell proliferation/neoplasm invasiveness/cell movement分类
医药卫生引用本文复制引用
沙亮,何剑波,李科志,陈闯,黄山,赵荫农,连芳,邬国斌..特异性下调LKB1的表达对肝癌细胞侵袭迁移能力的影响[J].临床肝胆病杂志,2017,33(5):875-879,5.基金项目
国家自然科学基金资助项目(81460426) （81460426）
国家自然科学基金资助项目(81360315) （81360315）
广西自然科学基金资助项目(2014GXNSFAA118191) （2014GXNSFAA118191）
