临床肝胆病杂志2017，Vol.33Issue(5)：932-936,5.DOI:10.3969/j.issn.1001-5256.2017.05.029
化痰祛湿活血方对非酒精性脂肪性肝炎大鼠ADPN/AMPK/ACC通路的影响
Effect of Huatan Qushi Huoxue prescription on the ADPN/AMPK/ACC signaling pathway in rats with nonalcoholic steatohepatitis
摘要
Abstract
Objective To investigate the effect of Huatan Qushi Huoxue prescription on the adiponectin (ADPN)/adenosine monophosphate -activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) signaling pathway in rats with nonalcoholic steatohepatitis (NASH).Methods A total of 72 male Sprague-Dawley rats were randomly divided into blank group,model group,control group,and high-,middle-,and low-dose Huatan Qushi Huoxue prescription groups.All rats except those in the blank group were given high-fat diet combined with intraperitoneal injection of tetracycline to establish a rat model of NASH.At week 2 after model establishment,the Huatan Qushi Huoxue prescription groups were treated with high-,middle-,and low-dose Huatan Qushi Huoxue prescription,and polyene phosphatidylcholine (Yishanfu) capsules were given as control.At the end of the experiment,the content of free fatty acid (FFA) in liver homoge-nate was measured,and ELISA and RT-qPCR were used to measure the protein and mRNA expression of ADPN,adiponectin receptor-2(AdipoR2),AMPK,ACC,and carnitine palmitoyltransferase-1 (CPT-1) in liver tissue.An analysis of variance was used for compari-son of data between multiple groups;the least significant difference t-test was used for comparison of data with homogeneity of variance be-tween two groups,and the Dunnett~ T3 method was used for comparison of data with heterogeneity of variance.Results Compared with the control group,the high-dose group had significant reductions in FFA level in liver homogenate (229.07 ± 46.62 μmol/gprot vs 382.75 ±30.93 μmol/gprot,P ＜ 0.01) and mRNA and protein expression of ACC in liver tissue (mRNA:1.66 ± 0.04 vs 2.40 ± 0.37,P ＜ 0.01;protein:512.20 ±60.70 pg/g vs 756.72 ±61.21 pg/g,P ＜0.01),as well as significant increases in the mRNA and protein expression of ADPN (mRNA:1.53 ±0.31 vs 0.75 ±0.11,P ＜0.01;protein:22.23 ±2.75 μg/g vs 14.01 ±2.17 μg/g,P ＜0.01),AdipoR2 (mRNA:1.38 ± 0.33 vs 0.61 ± 0.09,P ＜ 0.01;protein:128.41 ± 10.46 ng/g vs 94.62 ± 6.88 ng/g,P ＜ 0.01),AMPK (mRNA:1.43 ±0.39vs0.78±0.04,P＜0.01;protein:1353.79 ±59.52 μg/gvs 1107.19 ±56.78 μg/g,P＜0.01),andCPT-1 (mRNA:1.39±0.31 vs0.52±0.04,P＜0.01;protein:175.54±9.91 U/gvs 128.74±12.74 U/g,P＜0.01).Conclusion Huatan Qushi Huoxue prescription can effectively activate the ADPN/AMPK/ACC signaling pathway,which may affect the β oxidation and synthesis of fattY acids and reduce fat deposition in hepatocytes.关键词
非酒精性脂肪性肝炎/化痰祛湿活血方/脂联素/腺苷酸活化蛋白激酶/乙酰CoA羧化酶Key words
nonalcoholic steatohepatitis/Huata Qushi Huoxue recipe/adiponectin/amp-activated protein kinase/acetyl-CoA carboxylase分类
医药卫生引用本文复制引用
赵文霞,闫乐,邵明义,张丽慧..化痰祛湿活血方对非酒精性脂肪性肝炎大鼠ADPN/AMPK/ACC通路的影响[J].临床肝胆病杂志,2017,33(5):932-936,5.基金项目
河南省中医临床学科领军人才培育项目 （）
国家自然基金(81473651) （81473651）
