农业资源与环境学报2017，Vol.34Issue(3)：257-265,9.DOI:10.13254/j.jare.2017.0014
石油污染土壤中正十六烷降解菌的效果研究
Degradation Capability of n-hexadecane Degrading Bacteria from Petroleum Contaminated Soils
摘要
Abstract
Samplings were performed in the petroleum contaminated soils of Dongying,Shandong Province of China.Degrading bacteria was isolated through enrichment in a Bushnel-Hass medium,with n-hexadecane as the sole source of carbon and energy.Then the isolated strains were identified by amplification of 16S rDNA gene and sequencing.The strain TZSX2 was selected as the powerful bacteria with stronger degradation ability,which was then identified as Rhodococcus hoagii genera based on the constructing results of the phylogenetic tree.The optimum temperature that allowed both high growth and efficient degradation ratio was in the scope of 28 ～36 ℃,and gas chromatography results showed that approximately more than 30％ of n-hexadecane could be degraded in one week of incubation within the temperature range.Moreover,the strain TZSX2 was able to grow in high concentrations of n-hexadecane.The degradation rate reached 79％ when the initial n-hexadecane concentration was 2 mL· L-1,while it still achieved 12％ with n-hexadecane concentration of 20 mL· L-1.The optimal pH was 9 that allowed the highest growth and the greatest degradation rate of 91％.Above all,the screened strain TZSX2 showed high capabilities of alkali tolerance with excellent degradation efficiency for even high concentration of n-hexadecane,and thus it would be quite suitable for the remediation of petroleum contaminated soils especially in the extreme environment.关键词
石油污染土壤/正十六烷/降解菌/环境因子/降解率Key words
petroleum contaminated soil/n-hexadecane/degrading bacteria/environmental factors/degradation rate分类
资源环境引用本文复制引用
彭怀丽,李红娜,张丽,马金奉,阿旺次仁,唐哲仁,朱昌雄..石油污染土壤中正十六烷降解菌的效果研究[J].农业资源与环境学报,2017,34(3):257-265,9.基金项目
"韩国量子能公司农业用颗粒(丸)粉末及使用方法在中国农业生产中实用效果测试计划"[开(2015)1] （丸）
国家水体污染控制与治理科技重大专项(2014ZX07101-012-001) （2014ZX07101-012-001）
