药学研究2017，Vol.36Issue(5)：255-258,4.DOI:10.13506/j.cnki.jpr.2017.05.002
马粪海胆生殖腺中多糖的纯化方法及其抗肿瘤作用研究
Research on the purification methods and their antitumor function of the polysaccharides from Hemicentrotus pulcherrimus gonad
摘要
Abstract
Objective To optimize the purification methods of the polysaccharides from Hemicentrotus pulcherrimus gonad,and to evaluate their cytotoxic activity in vitro against human cancer cell lines.Methods The polysaccharides in H.pulcherrimus gonad were extracted with water,and the protein was removed by enzymic hydrolysis of papain and Sevage method,followed by dialyzed in flowing distilled water,precipitated in water and fully dried in progress.The total polysaccharide was separated by column chromatography on Sephacryl S-300 gel.The content of polysaccharides was measured with phenol-sulfuric acid method,and the antitumor effect was tested by MTT method.Results The optimum deproteinate effects were obtained by combination of enzymic hydrolysis of papain and Sevage method,and the optimized amount of papain was 100∶6 (the ration of the masses of defatted powder to papain),and the content of the purified total polysaccharide was 90%.The total polysaccharide and two separated polysaccharides showed anti-proliferative activity of Bel7402 cell lines.Conclusion The determined purification methods were applicable for the polysaccharides from H.pulcherrimus gonad for the simple and convenient operation,and that the purified polysaccharides displayed good physicochemical property and water-solubility.关键词
马粪海胆/纯化方法/多糖/抗肿瘤作用Key words
Hemicentrotus pulcherrimus/Purification methods/Polysaccharide/Antitumor effect分类
医药卫生引用本文复制引用
杜敏,陈琴,刘永俊,赵峰,侯桂革,王春华..马粪海胆生殖腺中多糖的纯化方法及其抗肿瘤作用研究[J].药学研究,2017,36(5):255-258,4.基金项目
山东省自主创新及成果转化专项(No.2014CGZH1316) （No.2014CGZH1316）
山东省医药卫生科技发展计划(No.2015WS0496) （No.2015WS0496）
