药学研究2017，Vol.36Issue(5)：259-261,286,4.DOI:10.13506/j.cnki.jpr.2017.05.003
液相色谱-串联质谱法测定大鼠血浆中左旋泮托拉唑钠的浓度及其毒代动力学研究
Determination of (S)-pantoprazole sodium in rat plasma and its toxicokinetics by LC-MS/MS
徐玲玲 1王芳 1刘爱明 1逯海燕 1贾庆文 1戚敏1
作者信息
- 1. 山东省药学科学院,山东 济南 250101
- 折叠
摘要
Abstract
Objective To establish an LC-MS/MS method to determine (S)-pantoprazole sodium in rat plasma and toxicokinetics.Methods After protein precipitation with acetonitrile,the analyte and internal standard were separated on Chiralcel OJ-RH column (4.6 mm×150 mm,5 μm),with acetonitrile-water (28∶72) as mobile phase at a flow 0.6 mL·min-1.Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode.The MRM transitions of m/z384.0-m/z199.8 and m/z180.0-m/z110.0 were used to quantify (S)-pantoprazole sodium and phenacetin.Results The calibration curve was linear over the concentration range of 50～30 000 ng·mL-1.RSD was less than 15%,and the accuracy was in the range of 85%～115%.Its AUC0～4 hand Cmax were directly to proportional to doses.Conclusion The validation results showed that the established method could be applied to the determination of (S)-pantoprazole sodium in plasma of rats,and was suitable to the toxicokinetics study.关键词
左旋泮托拉唑钠/大鼠血浆/液相色谱-串联质谱法/毒代动力学Key words
(S)-pantoprazole sodium/Rat plasma/LC-MS/MS/Toxicokinetics分类
医药卫生引用本文复制引用
徐玲玲,王芳,刘爱明,逯海燕,贾庆文,戚敏..液相色谱-串联质谱法测定大鼠血浆中左旋泮托拉唑钠的浓度及其毒代动力学研究[J].药学研究,2017,36(5):259-261,286,4.
