药学研究2017，Vol.36Issue(5)：266-269,4.DOI:10.13506/j.cnki.jpr.2017.05.005
基于UPLC-Q-TOF-MS法对中药山绿茶化学成分分析
Study on chemical constituents in Ilex hainanensis Merr.by UPLC-Q-TOF-MS
张丽敏 1王嘉仡 2孟宪生 3包永睿 1王帅 2李天娇1
作者信息
- 1. 辽宁中医药大学药学院,辽宁 大连 116600
- 2. 辽宁省现代研究工程试验室,辽宁 大连 116600
- 3. 辽宁省药品检验检测院,辽宁 沈阳 110023
- 折叠
摘要
Abstract
Objective To establish an analysis method for the determination of chemical constituents in Ilex hainanensis Merr.extract by UPLC-Q-TOF-MS.Methods An Agilent poroshell SB-C18 column(4.6 mm×50 mm,2.7 μm)was used with a gradient elution of acetonitrile-water containing 0.1% formic acid.Mass spectrometric data were obtained under electrospray inoization (ESI) negatie mode.With Agilent Qualitative Analysis software to extract and match of chromatographic peaks.Results Combined with reference,related literature and database analysis,27 compounds were identified by mass spectrometry information.Conclusion This method was fast,sensitive and comprehensive with the rapid identification of chemical constituents in the Ilex hainanensis Merr.extract,which would provide the evidences for clarifying the efficacy material base of the Ilex hainanensis Merr..关键词
山绿茶/超高效液相色谱串联四级杆飞行时间质谱/化学成分/质谱特征/碎片离子Key words
Ilex hainanensis Merr./UPLC-Q-TOF-MS/Chemical constituents/Mass spectrum characteristics/Frag-ment ions分类
医药卫生引用本文复制引用
张丽敏,王嘉仡,孟宪生,包永睿,王帅,李天娇..基于UPLC-Q-TOF-MS法对中药山绿茶化学成分分析[J].药学研究,2017,36(5):266-269,4.
